Studies in fat grafting: Part III. Fat grafting irradiated tissue--improved skin quality and decreased fat graft retention.
Studies in fat grafting: Part III. Fat grafting irradiated tissue--improved skin quality and decreased fat graft retention.
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DOI：
10.1097/prs.0000000000000326
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发表时间：
2014-08
影响因子：
3.6
通讯作者：
Wan DC
中科院分区：
文献类型：
--
作者：
Garza RM;Paik KJ;Chung MT;Duscher D;Gurtner GC;Longaker MT;Wan DC
放射治疗后，皮肤会纤维化，这对重建外科医生来说是一个难题。越来越多的人相信，在辐射皮肤下移植脂肪可以逆转辐射造成的损害。本研究评价了脂肪移植对辐照皮肤的影响，沿着脂肪移植质量和辐照组织中的保留率。9只成年Crl：NU-Foxn 1 nu CD-1小鼠接受30戈伊的头皮外部束辐射。辐射后四周，收获来自辐射和非辐射小鼠的头皮皮肤，并在组织学上比较真皮厚度、胶原含量和血管密度。然后将人脂肪移植物注射到头皮的皮下平面中。在移植后2周和8周对辐照组进行皮肤评估，并在基线和移植后每2周至8周使用微型CT测量脂肪移植物保留。最后，在8周时收集脂肪移植物样品，并进行质量评分。脂肪移植导致真皮厚度减少，胶原蛋白含量减少，并增加血管密度在照射皮肤。CT分析显示，与非辐照组相比，辐照组的脂肪移植物存活率显著降低。组织学评分显示辐照组和非辐照组之间的脂肪移植物质量无差异。脂肪移植减轻放射性纤维化的真皮胶原沉积和血管损耗。虽然脂肪移植物保留率显着低于辐照比非辐照组织，保留的脂肪组之间的质量是相似的。
Following radiation therapy, skin becomes fibrotic and can present a difficult problem for reconstructive surgeons. There is an increasing belief that fat grafting under radiated skin can reverse the damage caused by radiation. The present study evaluated the effect of fat grafting on irradiated skin, along with fat graft quality and retention rates in irradiated tissue. Nine adult Crl:NU-Foxn1nu CD-1 mice received 30 Gy external beam radiation of the scalp. Four weeks following radiation, scalp skin from irradiated and non-irradiated mice was harvested and compared histologically for dermal thickness, collagen content, and vascular density. Human fat grafts were then injected in the subcutaneous plane of the scalp. Skin assessment was performed in the irradiated group at 2 and 8 weeks post-grafting, and fat graft retention was measured at baseline and every 2 weeks up to 8 weeks post-grafting using micro-CT. Finally, fat graft samples were explanted at 8 weeks, and quality scoring was performed. Fat grafting resulted in decreased dermal thickness, decreased collagen content, and increased vascular density in irradiated skin. CT analysis revealed significantly decreased fat graft survival in the irradiated group when compared to the non-irradiated group. Histological scoring of explanted fat grafts demonstrated no difference in quality between the irradiated and non-irradiated groups. Fat grafting attenuates dermal collagen deposition and vessel depletion characteristic of radiation fibrosis. Although fat graft retention rates are significantly lower in irradiated than non-irradiated tissue, the quality of retained fat between the groups is similar.