Addressing the Analytical Challenges for the Detection of Ciguatoxins Using an Electrochemical Biosensor
Addressing the Analytical Challenges for the Detection of Ciguatoxins Using an Electrochemical Biosensor
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DOI：
10.1021/acs.analchem.9b04499
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发表时间：
2020-04-07
影响因子：
7.4
通讯作者：
Campas, Monica
中科院分区：
文献类型：
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作者：
Leonardo, Sandra;Gaiani, Greta;Campas, Monica
雪卡毒素（CTX）在海鲜安全中的重要性及其在远离热带地区的地方出现的情况凸显了需要简单、低成本的方法来敏感、快速地检测这些强效海洋毒素，以保护海鲜消费者。本文提出了一种用于检测CTX的电化学免疫传感器。提出了一种夹心结构，使用磁珠（MB）作为两种捕获抗体的固定化支持物，其组合有利于检测CTX 1B，CTX 3C，54-脱氧CTX 1B和51-羟基CTX 3C。聚HRP-链霉亲和素用于检测生物素化检测抗体。首先使用比色法优化实验条件，随后将这些条件用于电极阵列上的电化学检测。CTX 1B和5 1-羟基CTX 3C达到pg/mL水平的检测限。的免疫传感器的鱼样品的分析的适用性进行了证明，实现检测CTX 1B的含量低至0.01 μ g/kg，并提供与使用小鼠生物测定（MBA）和细胞为基础的测定（CBA），并确认液相色谱法耦合到高分辨率质谱（LC-ESI-HRMS）得到的结果相关。这一方便用户的生物分析工具可用于快速检测CTX，可用于降低雪卡毒风险，并有助于保护消费者健康。
The importance of ciguatoxins (CTXs) in seafood safety and their emerging occurrence in locations far away from tropical areas highlight the need for simple and low-cost methods for the sensitive and rapid detection of these potent marine toxins to protect seafood consumers. Herein, an electrochemical immunosensor for the detection of CTXs is presented. A sandwich configuration is proposed, using magnetic beads (MBs) as immobilization supports for two capture antibodies, with their combination facilitating the detection of CTX1B, CTX3C, 54-deoxyCTX1B, and 51-hydroxyCTX3C. PoIyHRP-streptavidin is used for the detection of the biotinylated detector antibody. Experimental conditions are first optimized using colorimetry, and these conditions are subsequently used for electrochemical detection on electrode arrays. Limits of detection at the pg/mL level are achieved for CTX1B and 5 1-hydroxyCTX3C. The applicability of the immunosensor to the analysis of fish samples is demonstrated, attaining detection of CTX1B at contents as low as 0.01 mu g/kg and providing results in correlation with those obtained using mouse bioassay (MBA) and cell-based assay (CBA), and confirmed by liquid chromatography coupled to high-resolution mass spectrometry (LC-ESI-HRMS). This user-friendly bioanalytical tool for the rapid detection of CTXs can be used to mitigate ciguatera risk and contribute to the protection of consumer health.