Studies of the constitutive expression of the mouse erythropoietin gene.
Studies of the constitutive expression of the mouse erythropoietin gene.
复制标题
小鼠促红细胞生成素基因组成型表达的研究。
DOI：
10.1111/j.1749-6632.1989.tb22406.x
复制
发表时间：
1989
影响因子：
5.2
通讯作者：
Goldwasser,E
中科院分区：
文献类型：
--
作者：
Beru,N;McDonald,J;Goldwasser,E
IW32和NN10细胞系是结构性分泌促红细胞生成素(EPO)的红白血病细胞系。IW32细胞除了正常基因外，还有一个重排和扩增的EPO基因。NN10细胞只含有正常的EPO基因。DNase I超敏反应研究表明，在IW32细胞中，转录活性最高的是重排基因。对重排的EPO基因上游区域的序列分析表明，重排引入了一个接近EPO基因的转录活性基因。这种并列可能解释了EPO基因在重排座位上的转录激活。
The IW32 and NN10 cell lines are erythroleukemic cell lines which secrete erythropoietin (epo) into the culture medium constitutively. IW32 cells have a rearranged and amplified epo gene in addition to the normal gene. NN10 cells contain only the normal epo gene. DNase I hypersensitivity studies suggested that, in IW32 cells, it is the rearranged gene which is transcriptionally active. Sequence analysis of the upstream region of the rearranged epo gene suggests that the rearrangement has served to introduce a transcriptionally active gene close to the epo gene. This juxtaposition may explain the transcriptional activation of the epo gene at the rearranged locus.