Expression of circadian clock genes in retinal dopaminergic cells
Expression of circadian clock genes in retinal dopaminergic cells
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DOI：
10.1017/s0952523807070538
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发表时间：
2007-07-01
影响因子：
1.9
通讯作者：
Raviola, Elio
中科院分区：
文献类型：
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作者：
Dorenbos, Ronald;Contini, Massimo;Raviola, Elio
哺乳动物的神经视网膜包含单个或多个内在昼夜节律振荡器，可以直接由光周期携带。多巴胺能无分泌（DA）细胞是视网膜振荡器的一个特别有趣的候选者，因为多巴胺在光适应中的关键作用，以及多巴胺受体在视网膜中的广泛分布。通过单细胞终点RT-PCR，我们发现视网膜DA细胞含有六个生物钟核心成分的转录本：Bmal1、clock、Cry1、Cry2、Per1和Per2。杆状光感受器代表阴性对照，因为它们似乎不包含时钟转录本。通过比较野生型和Bmal1（-/-）小鼠视网膜DA细胞细胞核的免疫染色，我们最终证实DA细胞含有Bmall基因编码的蛋白。因此，DA细胞可能包含一个生物钟，可以预测视网膜照明的可预测变化。
The mammalian neural retina contains single or multiple intrinsic circadian oscillators that can be directly entrained by light cycles. Dopaminergic amacrine (DA) cells represent an especially interesting candidate as a site of the retinal oscillator because of the crucial role of dopamine in light adaptation, and the widespread distribution of dopamine receptors in the retina. We hereby show by single-cell, end-point RT-PCR that retinal DA cells contain the transcripts for six core components of the circadian clock: Bmal1, Clock, Cry1, Cry2, Per1, and Per2. Rod photoreceptors represented a negative control, because they did not appear to contain clock transcripts. We finally confirmed that DA cells contain the protein encoded by the Bmall gene by comparing immunostaining of the nuclei of DA cells in the retinas of wildtype and Bmal1(-/-) mice. It is therefore likely that DA cells contain a circadian clock that anticipates predictable variations in retinal illumination.