Visible light-induced antibacterial effects of the luminescent complex of hydroxyapatite and 8-hydroxyquinoline with gray titania coating
Visible light-induced antibacterial effects of the luminescent complex of hydroxyapatite and 8-hydroxyquinoline with gray titania coating
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DOI：
10.1016/j.apsusc.2018.04.106
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发表时间：
2018-08
影响因子：
6.7
通讯作者：
Takehiko Matsuya;S. Morakul;Y. Otsuka;K. Ohnuma;M. Tagaya;S. Motozuka;Y. Miyashita;Y. Mutoh
中科院分区：
文献类型：
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作者：
Takehiko Matsuya;S. Morakul;Y. Otsuka;K. Ohnuma;M. Tagaya;S. Motozuka;Y. Miyashita;Y. Mutoh
本研究的目的是观察羟基磷灰石与8-羟基喹啉的发光络合物在灰色二氧化钛涂层上的可见光诱导抗菌效果。等离子喷涂羟基磷灰石(HAP)涂层广泛应用于牙种植体牙槽骨之间的粘接。然而，最近有关于种植体表面细菌感染的报道，传统的抗菌技术不能长期使用。因此，提出了一种利用8-羟基磷灰石和8-羟基喹啉(8-HQ)与灰色二氧化钛形成的可被可见光活化的新技术。采用等离子喷涂技术制备了可见光敏感的二氧化钛涂层。用拉曼光谱、傅里叶变换红外光谱和X射线衍射仪对等离子喷涂的HAP/TiO2进行了表征。通过光密度(OD)、菌落形成单位(CFU)和荧光显微镜观察，评价其光催化活性和抗菌性能。等离子喷涂的二氧化钛粉末虽然在可见光范围内具有吸收性能，但其颜色仍为深灰色，但其物相转变为金红石型的二氧化钛。等离子喷涂后的二氧化钛粉末被称为灰色二氧化钛，然后用三种不同的激光(蓝色、绿色和红色)照射灰色二氧化钛产生1O2。抗菌实验表明，复合体的存在和发光二极管的照射均能显著降低细胞的集落形成单位(CFU)数。此外，HAP-8HQ复合体即使在没有光照射的情况下也能降低大肠杆菌的粘附性。灰色二氧化钛涂层表面羟基磷灰石的抗菌性能的增强可以归因于荧光增强了灰色二氧化钛表面的光的功率密度。
The aim of this study is to observe visible light-induced antibacterial effects of the luminescent complex of hydroxyapatite and 8-hydroxyquinoline with gray titania coating. Plasma-sprayed Hydroxyapatite (HAp) coating has been widely used as bonding between alveolar bones with dental implants. However, bacterial infection on the surfaces of dental implants has recently been reported and conventional antibacterial technologies cannot last long-term use. Therefore a novel technology using the complex of hydroxyapatite and 8-hydroxyquinoline (8-Hq) with gray titania was proposed, which can be activated by visible light. Ti 2 O 3 powder was used in a plasma-spraying process to fabricate visible-light-sensitive titania coating. The plasma-sprayed HAp/Ti 2 O 3 was characterized by Raman Spectroscopy, FTIR and XRD. Its photo catalytic activity was evaluated using chemiluminescence observation and antibacterial property was evaluated by optical density measurement (OD), colony forming unit (CFUs) and fluorescent microscope observation. The plasma-sprayed Ti 2 O 3 powder revealed that its phase changed to TiO 2 (Rutile) though the color of the plasma-sprayed Ti 2 O 3 powder kept dark gray, which possessed its absorbency in visible-light region. The plasma-sprayed Ti 2 O 3 powder was then called gray titania and 1 O 2 was generated from the gray titania by irradiating three types of laser (blue, green and red). Antibacterial evaluation revealed that both the existence of the complex and the irradiation of LEDs could significantly decrease the numbers of colony forming unit (CFUs). Furthermore, HAp-8Hq complex decreased adhesion of E. coli even without light irradiation. An enhancement in the antibacterial property of HAp complexes with gray titania coating can be attributed to an increased power density of light on the surface of gray titania by fluorescence.