Expression of TRPC homologs in endothelial cells and smooth muscle layers of human arteries
Expression of TRPC homologs in endothelial cells and smooth muscle layers of human arteries
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DOI：
10.1007/s00418-004-0720-y
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发表时间：
2004-11
影响因子：
2.3
通讯作者：
Ham Yip;W. Chan;P. Leung;H. Kwan;Cuiling Liu;Yu Huang;Villaz Michel;D. Yew;X. Yao
中科院分区：
文献类型：
--
作者：
Ham Yip;W. Chan;P. Leung;H. Kwan;Cuiling Liu;Yu Huang;Villaz Michel;D. Yew;X. Yao
TRPC channels are a group of Ca2+-permeable nonselective cation channels that mediate store-operated and/or agonist-stimulated Ca2+influx in a variety of cell types. In this study, we extensively examined the expression patterns of TRPC homologs in human vascular tissues. RT-PCR amplified cDNA fragments of TRPC1 (505 bp), TRPC3 (372 bp), TRPC4 (499 bp), TRPC5 (325 bp), TRPC6 (509 bp), and TRPC7 (187 bp) from RNA isolated from cultured human coronary artery endothelial cells. In situ hybridization yielded strong labeling of TRPC1,3–6 in the endothelial and smooth muscle cells of human coronary and cerebral arteries. TRPC7 labeling was exclusively found in endothelial cells but not in smooth muscle cells. Results from immunohistochemical staining were consistent with those from in situ hybridization. Similar expression patterns of TRPC homologs were also observed in arterioles and vaso vasora. In conclusion, our study indicates that TRPC homologs are widely expressed in human vessels of all calibers, including medium-sized coronary arteries and cerebral arteries, smaller-sized resistance arteries, and vaso vasora. These results suggest a ubiquitous role of TRPC homologs in regulating blood supply to different regions and in controlling arterial blood pressure.
