Inflorescence abscission protein SlIDL6 promotes low light intensityinduced tomato flower abscission.
Inflorescence abscission protein SlIDL6 promotes low light intensityinduced tomato flower abscission.
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DOI：
10.1093/plphys/kiab121
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发表时间：
2021-03
期刊：
影响因子：
7.4
通讯作者：
Ruizhen Li;C. Shi;Xiaoyang Wang;Y. Meng;Lina Cheng;Caizhong Jiang;M. Qi;Tao Xu;Tianlai Li
中科院分区：
文献类型：
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作者：
Ruizhen Li;C. Shi;Xiaoyang Wang;Y. Meng;Lina Cheng;Caizhong Jiang;M. Qi;Tao Xu;Tianlai Li
在许多有果植物物种中，花的脱落是由弱光胁迫引起的。在这里，我们阐明了由多肽花序脱落缺陷（IDA）介导的信号传导如何控制番茄（Solanum lycopersicum）弱光诱导的落花。我们分析了ida样基因（SlIDL6）在弱光诱导花脱落过程中的表达模式，并使用串联质谱法对SlIDL6成熟肽进行了鉴定和表征。为了研究SlIDL6在体内的功能，我们建立了番茄敲除系。此外，利用酵母单杂交实验研究了SlWRKY17转录因子与SlIDL6启动子的结合，以及SlWRKY17表达的沉默延迟了弱光诱导的花脱落。SlIDL6在脱落区特异表达，在弱光诱导脱落和乙烯处理时表达量较高。SlIDL6敲除系弱光诱导落花延迟，SlIDL6肽的应用加速了脱落。SlIDL6的过表达挽救了拟南芥（Arabidopsis thaliana）中ida突变体的表型，表明物种之间的功能保护。slidl6介导的脱落是通过一个不依赖乙烯的途径。我们报道了一个SlWRKY17- SlIDL6调节模块，该模块通过增加参与细胞壁重塑和拆卸的酶的表达，在弱光下促进脱落。
In many fruiting plant species, flower abscission is induced by low light stress. Here, we elucidated how signaling mediated by the peptide INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) controls low light-induced flower drop in tomato (Solanum lycopersicum). We analyzed the expression patterns of an IDA-Like gene (SlIDL6) during low light-induced flower abscission, and used tandem mass spectrometry to identify and characterize the mature SlIDL6 peptide. Tomato knockout lines were created to investigate the in vivo function of SlIDL6. In addition, yeast one-hybrid assays were used to investigate the binding of the SlWRKY17 transcription factor to the SlIDL6 promoter, and silencing of SlWRKY17 expression delayed low light-induced flower abscission. SlIDL6 was specifically expressed in the abscission zone (AZ) and at high levels during low light-induced abscission and ethylene treatment. SlIDL6 knockout lines showed delayed low light-induced flower drop, and the application of SlIDL6 peptide accelerated abscission. Overexpression of SlIDL6 rescued the ida mutant phenotype in Arabidopsis (Arabidopsis thaliana), suggesting functional conservation between species. SlIDL6-mediated abscission was via an ethylene-independent pathway. We report a SlWRKY17- SlIDL6 regulatory module that functions in low light promoted abscission by increasing the expression of enzymes involved in cell wall remodeling and disassembly.