Reduced antigen‐presenting function of human Epstein‐Barr virus (EBV)‐B cells and monocytes after UVB radiation is accompanied by decreased expression of B7, intercellular adhesion molecule‐1 (ICAM‐1) and LFA‐3
Reduced antigen‐presenting function of human Epstein‐Barr virus (EBV)‐B cells and monocytes after UVB radiation is accompanied by decreased expression of B7, intercellular adhesion molecule‐1 (ICAM‐1) and LFA‐3
复制标题
UVB 辐射后人 Epstein-Barr 病毒 (EBV)-B 细胞和单核细胞的抗原呈递功能降低，伴随着 B7、细胞间粘附分子-1 (ICAM-1) 和 LFA-3 表达的降低
DOI：
10.1111/j.1365-2249.1995.tb03135.x
复制
发表时间：
1995
影响因子：
4.6
通讯作者：
M. Teunissen
中科院分区：
文献类型：
--
作者：
I. Kremer;J. D. Bos;M. Teunissen
在这项研究中，研究了紫外线B（UVB）辐射对抗原呈递功能的影响，以研究UVB是否通过共同机制抑制抗原呈递细胞（APC）。使用两种类型的人APC：EBV-B细胞和单核细胞，并且这些在体外用单次低剂量的UVB（范围0-200 J/m2）照射。当通过同种异体混合白细胞反应（MLR）或白色念珠菌或破伤风类毒素特异性T细胞反应测定时，EBV B细胞或单核细胞的辐照导致APC功能的剂量依赖性降低。我们的研究表明，APC功能的降低不太可能是由抗原加工或细胞因子产生的改变引起的。然而，UVB照射的APC显示粘附分子表达的显著变化。照射后的B细胞显示ICAM-1（30%）、LFA-3（25%）和B7 - 1（35%）表达降低，而HLA-DR、CD 19和LFA-1的白色表达不受影响。UVB照射单核细胞确实导致HLA-DR（30%），LFA-3（40%），ICAM-1（65%）和B7 - 1和B7 - 3（90%）的表达降低，但对CD 14，LFA-1和ICAM-3表达无影响。加入未照射的细胞（但不是这些细胞的上清液）或CD 28抗体部分恢复了T细胞活化，表明UVB诱导的APC功能降低至少部分是通过损害共刺激分子表达介导的。
In this study, the effect of ultraviolet‐B (UVB) radiation on antigen‐presenting function was studied, to investigate whether antigen‐presenting cells (APC) are inhibited by UVB through a common mechanism. Two types of human APC were used: EBV‐B cells and monocytes, and these were irradiated in vitro with single low doses of UVB (range 0–200 J/m2). Irradiation of EBV‐B cells or monocytes resulted in similar dose‐dependent reduction in APC function, when determined by the allogeneic mixed leucocyte reaction (MLR) or Candida albicans‐ or tetanus toxoid‐specific T cell response. Our study shows that the reduced APC function was not likely to be caused by alterations in antigen processing or cytokine production. However, UVB‐irradiated APC displayed marked changes in adhesion molecule expression. Irradiated EBV‐B cells showed reduced expression of ICAM‐1 (30%), LFA‐3 (25%) and B7‐1 (35%), white expression of HLA‐DR, CD19 and LFA‐1 was not affected. UVB irradiation of monocytes did result in reduction in the expression of HLA‐DR (30%), LFA‐3 (40%), ICAM‐1 (65%) and B7‐1 and B7‐3 (90%), but had no effect on CD14, LFA‐1 and ICAM‐3 expression. Addition of non‐irradiated cells (but not the supernatant of these cells) or CD28 antibodies partly restored T cell activation, indicating that UVB‐induced reduction in APC function is at least partly mediated via impairment of co‐stimulatory molecule expression.
登录
查看更多内容
DOI：
10.1172/jci114600
发表时间：
1990-05
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Jean Krutmann;Islam U. Khan;Robert S. Wallis;Fen Zhang;Elizabeth A. Rich;J. Ellner;C. A. Elmets
通讯作者：
Jean Krutmann;Islam U. Khan;Robert S. Wallis;Fen Zhang;Elizabeth A. Rich;J. Ellner;C. A. Elmets
DOI：
10.1111/1523-1747.ep12504877
发表时间：
1990-11
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
T. Yoshikawa;V. Rae;W. Bruins-Slot;J. V. D. Berg;J. Taylor;J. Taylor;J. Streilein
通讯作者：
T. Yoshikawa;V. Rae;W. Bruins-Slot;J. V. D. Berg;J. Taylor;J. Taylor;J. Streilein
DOI：
10.1073/pnas.90.14.6586
发表时间：
1993-07-15
影响因子：
11.1
作者：
GIMMI, CD;FREEMAN, GJ;NADLER, LM
通讯作者：
NADLER, LM
T 细胞增殖的辅助细胞刺激需要主动抗原处理、Ia 限制性抗原呈递和单独的非特异性第二信号。
DOI：
--
发表时间：
1981
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Germain,RN
通讯作者：
Germain,RN
紫外线 B 辐射将朗格汉斯细胞从免疫原性转化为耐受性抗原呈递细胞。
DOI：
--
发表时间：
1991
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Simon,JC;Tigelaar,RE;Bergstresser,PR;Edelbaum,D;CruzJr,PD
通讯作者：
CruzJr,PD