ANALYSIS OF PRODUCTS FORMED DURING BLEOMYCIN-MEDIATED DNA-DEGRADATION
ANALYSIS OF PRODUCTS FORMED DURING BLEOMYCIN-MEDIATED DNA-DEGRADATION
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DOI：
10.1021/bi00342a008
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发表时间：
1985-01-01
期刊：
影响因子：
2.9
通讯作者：
HECHT, SM
中科院分区：
文献类型：
--
作者：
MURUGESAN, N;XU, C;HECHT, SM
通过使用DNA，确定核苷酸组成的共聚物，以及在5' -和3' -末端附近具有假定的博来霉素切割位点的合成十二核苷酸，分离出伴随DNA链断裂形成的产物，并通过与真实合成样品的直接比较进行结构鉴定和定量。铁(II)-博莱霉素切割DNA链的产物包括在其3 "-末端具有四种可能的核苷3 "-（磷酸-2"- o-糖基酸酯）中的每一种的寡核苷酸，以及四种可能的基丙烯。至少对于3-（腺素-9”-基）原体和3-（胸腺素-1-基）原体，形成的产物完全是反式构型。
By the use of DNA, copolymers of defined nucleotide composition, and a synthetic dodecanucleotide having putative bleomycin cleavage sites in proximity to the 5''- and 3''-termini, the products formed concomitant with DNA strand scission have been isolated and subjected to structural identification and quantitation via direct comparison with authentic synthetic samples. The products of DNA strand scission by Fe(II)-bleomycin include oligonucleotides having each of the four possible nucleoside 3''-(phosphoro-2"-O-glycolates) at their 3''-termini, as well as the four possible base propenals. At least for 3-(adenin-9''-yl)propenal and 3-(thymin-1-yl)propenal, the products formed were exclusively of the trans configuration.