The "Duckweed Dip": Aquatic Spirodela polyrhiza Plants Can Efficiently Uptake Dissolved, DNA-Wrapped Carbon Nanotubes from Their Environment for Transient Gene Expression.
The "Duckweed Dip": Aquatic Spirodela polyrhiza Plants Can Efficiently Uptake Dissolved, DNA-Wrapped Carbon Nanotubes from Their Environment for Transient Gene Expression.
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“浮萍浸”：水生紫萍植物可以有效地从其环境中摄取溶解的、DNA 包裹的碳纳米管，用于瞬时基因表达。
DOI：
10.1101/2023.08.21.554121
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Josephs,EricA
中科院分区：
文献类型：
--
作者：
Islam,Tasmia;Kalkar,Swapna;Tinker-Kulberg,Rachel;Ignatova,Tetyana;Josephs,EricA
Duckweeds (Lemnaceae) are aquatic nongrass monocots that are the smallest and fastest-growing flowering plants in the world. While having simplified morphologies, relatively small genomes, and many other ideal traits for emerging applications in plant biotechnology, duckweeds have been largely overlooked in this era of synthetic biology. Here, we report that Greater Duckweed (Spirodela polyrhiza), when simply incubated in a solution containing plasmid-wrapped carbon nanotubes (DNA-CNTs), can directly uptake the DNA-CNTs from their growth media with high efficiency and that transgenes encoded within the plasmids are expressed by the plants─without the usual need for large doses of nanomaterials or agrobacterium to be directly infiltrated into plant tissue. This process, called the “duckweed dip”, represents a streamlined, “hands-off” tool for transgene delivery to a higher plant that we expect will enhance the throughput of duckweed engineering and help to realize duckweed’s potential as a powerhouse for plant synthetic biology.
