An Electron Tomographic Analysis of Giantin-Deficient Golgi Proposes a New Function of the Golgin Protein Family
An Electron Tomographic Analysis of Giantin-Deficient Golgi Proposes a New Function of the Golgin Protein Family
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巨蛋白缺陷高尔基体的电子断层扫描分析提出了高尔金蛋白家族的新功能
DOI：
10.1007/978-1-0716-2639-9_15
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发表时间：
2023
影响因子：
--
通讯作者：
Nishino Kunihiko
中科院分区：
文献类型：
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作者：
Satoh Ayano;Hayashi-Nishino Mitsuko;Nishino Kunihiko
高尔基体是介导蛋白质和脂质的修饰、分选和运输的细胞器。Golgins是一组具有卷曲螺旋结构的蛋白质，其定位于高尔基体，并且被认为起栓系物的作用以促进囊泡、Rab GTP酶和细胞骨架组分与高尔基体堆叠的对接。Giantin是最长的golgin，被认为与其他golgin（例如p115和GM 130）一起沿着COPI囊泡。与我们的预期相反，系链的丢失将导致细胞质中未系链的COPI囊泡增加，我们的电子显微镜观察显示，Giantin敲低后，高尔基体池中通常存在的窗孔减少。我们还发现，这种结构变化伴随着货物蛋白分泌和细胞表面糖基化的改变。这些结果表明Giantin的缺失引起的高尔基体结构变化与高尔基体功能之间存在相关性。在这里，我们描述了电子断层扫描方法检测高尔基体的结构变化。
The Golgi apparatus is an organelle that mediates modifications, sorting, and transport of proteins and lipids. Golgins are a group of proteins with coiled-coil structures that localize to the Golgi and are thought to function as tethers to facilitate the docking of vesicles, Rab GTPases, and cytoskeleton components to the Golgi stack. Giantin is the longest golgin and has been thought to function as a tether for COPI vesicles along with other golgins, such as p115 and GM130. Contrary to our expectation that the loss of the tether will result in an increase in untethered COPI vesicles in the cytoplasm, our electron microscopy observations showed that the fenestrae normally present in Golgi cisternae were reduced upon Giantin knockdown. We also found that this structural change is accompanied by altered secretion of cargo proteins and cell surface glycosylation. These results indicate that there exists a correlation between Golgi structural changes caused by the loss of Giantin and Golgi function. Here, we describe electron tomography methods for the detection of structural changes in the Golgi.