Detection of Antigens Reactive to IgE and IgA during Wheat Seed Maturation and in Different Wheat Cultivars
Detection of Antigens Reactive to IgE and IgA during Wheat Seed Maturation and in Different Wheat Cultivars
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小麦种子成熟过程中和不同小麦品种中与 IgE 和 IgA 反应的抗原的检测
DOI：
10.1159/000199713
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发表时间：
2009
影响因子：
2.8
通讯作者：
R. Valenta
中科院分区：
文献类型：
--
作者：
C. Constantin;A. Touraev;E. Heberle‐Bors;S. Quirce;W. Huber;R. Valenta
背景：饮食中摄入小麦可引起IgE介导的食物过敏和乳糜泻患者的超敏反应。目的：研究小麦种子成熟过程中IgE和IgA反应性抗原在不同小麦品种中的表达。方法：夏小麦在温室中生长，在规定的成熟阶段收获种子。成熟种子从13个不同定义的品种获得。蛋白质提取物制备不同的成熟阶段和品种的标准化程序的基础上，种子重量和分析IgE和伊加免疫印迹使用临床定义的患者患有小麦过敏或腹腔疾病的血清。结果：除了少数例外，IgE和IgA反应性小麦抗原的表达在小麦种子成熟过程中增加。小麦品种可以确定，其中某些IgE和IgA反应性成分的表达强烈减少或检测不到。结论：IgE和IgA反应性抗原的表达依赖于小麦种子的成熟，并在不同的小麦品种。
Background: Dietary intake of wheat causes hypersensitivity reactions in patients suffering from IgE-mediated food allergy and coeliac disease. Aim: To study the expression of IgE- and IgA-reactive antigens during wheat seed maturation and in different wheat cultivars. Methods: Summer wheat was grown in a glasshouse and seeds were harvested at defined maturation stages. Mature seeds were obtained from 13 different defined cultivars. Protein extracts were prepared from different maturation stages and cultivars with a standardized procedure based on seed weight and analysed by IgE and IgA immunoblotting using sera from clinically defined patients suffering from wheat allergy or coeliac disease. Results: With a few exceptions the expression of IgE- and IgA-reactive wheat antigens increased during wheat seed maturation. Wheat cultivars could be identified in which the expression of certain IgE- and IgA-reactive components was strongly reduced or not detectable. Conclusions: The expression of IgE- and IgA-reactive antigens depends on wheat seed maturation and varies in different wheat cultivars.