Small GTPase Rab39A interacts with UACA and regulates the retinoic acid-induced neurite morphology of Neuro2A cells
Small GTPase Rab39A interacts with UACA and regulates the retinoic acid-induced neurite morphology of Neuro2A cells
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DOI：
10.1016/j.bbrc.2013.04.051
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发表时间：
2013-05-24
影响因子：
3.1
通讯作者：
Fukuda, Mitsunori
中科院分区：
文献类型：
--
作者：
Mori, Yasunori;Matsui, Takahide;Fukuda, Mitsunori
我们以GTP锁定的Rab39A/B为诱饵，通过酵母双杂交试验筛选到Rab39A/B的特异性效应物，并确定UACA(带有卷曲结构域和锚蛋白重复序列的葡萄膜自身抗原)是一种特异性的Rab39A/B结合蛋白。缺失分析表明，UACA的C末端螺旋卷曲结构域是一个依赖GTP的Rab39结合域。ShRNA介导的内源性Rab39A或UACA在小鼠神经母细胞瘤Neuro2A细胞中的敲除导致维甲酸诱导的神经突起形态从多极形态转变为双极形态。综上所述，这些发现表明UACA在维甲酸诱导的Neuro2A细胞分化过程中发挥了Rab39A效应。(C)2013 Elsevier Inc.保留所有权利。
We screened for a Rab39-specific effector by performing a yeast two-hybrid assay with GTP-locked Rab39A/B as the bait and identified UACA (uveal autoantigen with coiled-coil domains and ankyrin repeats) as a specific Rab39A/B-binding protein. Deletion analysis revealed that a C-terminal coiled-coil domain of UACA functions as a GTP-dependent Rab39-binding domain. shRNA-mediated knockdown of endogenous Rab39A or UACA in mouse neuroblastoma Neuro2A cells resulted in a change in retinoic acid-induced neurite morphology from a multipolar morphology to a bipolar morphology. Taken together, these findings indicate that UACA functions as a Rab39A effector in the retinoic acid-induced differentiation of Neuro2A cells. (C) 2013 Elsevier Inc. All rights reserved.