Small intestinal stem cell identity is maintained with functional Paneth cells in heterotopically grafted epithelium onto the colon.
Small intestinal stem cell identity is maintained with functional Paneth cells in heterotopically grafted epithelium onto the colon.
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小肠干细胞与异位移植到结肠上皮中的功能性潘氏细胞保持同一性。
DOI：
10.1101/gad.245233.114
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发表时间：
2014-08-15
影响因子：
10.5
通讯作者：
Nakamura T
中科院分区：
文献类型：
--
作者：
Fukuda M;Mizutani T;Mochizuki W;Matsumoto T;Nozaki K;Sakamaki Y;Ichinose S;Okada Y;Tanaka T;Watanabe M;Nakamura T
为了开发用于小肠（SI）疾病的干细胞疗法，确定培养中的SI干细胞是否保留其组织再生能力是至关重要的。Fukuda等人证明，培养的鼠S1上皮类器官能够在结肠中重建自我更新的上皮细胞。SI衍生的移植物保持了许多仅SI独有的特征。这项研究提供的证据表明，培养的成体SI干细胞可能是一个来源的细胞治疗肠道疾病，保持其身份沿着胃肠道通过上皮细胞的内在机制。为了开发用于小肠（SI）疾病的干细胞疗法，确定培养中的SI干细胞是否保留其组织再生能力是至关重要的。通过使用异位移植方法，我们表明培养的小鼠SI上皮类器官能够在结肠中重建自我更新的上皮细胞。当稳定整合时，SI衍生的移植物显示出许多仅对SI独特但不同于结肠上皮的特征。我们的研究提供的证据表明，培养的成体SI干细胞可能是一个来源的细胞治疗肠道疾病，保持其身份沿着胃肠道通过上皮细胞的内在机制。
To develop stem cell therapy for small intestinal (SI) diseases, it is essential to determine whether SI stem cells in culture retain their tissue regeneration capabilities. Fukuda et al. demonstrate that cultured murine SI epithelial organoids are able to reconstitute self-renewing epithelia in the colon. The SI-derived grafts maintain many features unique only to the SI. This study provides evidence that cultured adult SI stem cells could be a source for cell therapy of intestinal diseases, maintaining their identity along the gastrointestinal tract through an epithelium-intrinsic mechanism. To develop stem cell therapy for small intestinal (SI) diseases, it is essential to determine whether SI stem cells in culture retain their tissue regeneration capabilities. By using a heterotopic transplantation approach, we show that cultured murine SI epithelial organoids are able to reconstitute self-renewing epithelia in the colon. When stably integrated, the SI-derived grafts show many features unique only to the SI but distinct from the colonic epithelium. Our study provides evidence that cultured adult SI stem cells could be a source for cell therapy of intestinal diseases, maintaining their identity along the gastrointestinal tract through an epithelium-intrinsic mechanism.
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影响因子：
4.4
作者：
Anderle, P;Sengstag, T;Mutch, DM;Rumbo, M;Praz, V;Mansourian, R;Delorenzi, M;Williamson, G;Roberts, MA
通讯作者：
Roberts, MA
影响因子：
64.8
作者：
Hao, Huai-Xiang;Xie, Yang;Cong, Feng
通讯作者：
Cong, Feng
影响因子：
29.4
作者：
Farin, Henner F.;Van Es, Johan H.;Clevers, Hans
通讯作者：
Clevers, Hans
影响因子：
64.8
作者：
Koo, Bon-Kyoung;Spit, Maureen;Clevers, Hans
通讯作者：
Clevers, Hans
影响因子：
29.4
作者：
Gregorieff, A;Pinto, D;Clevers, H
通讯作者：
Clevers, H