Cloning of the Lentinula edodes B mating-type locus and identification of the genetic structure controlling B mating
Cloning of the Lentinula edodes B mating-type locus and identification of the genetic structure controlling B mating
复制标题
香菇B交配型基因座的克隆及控制B交配的遗传结构鉴定
DOI：
10.1016/j.gene.2013.08.090
复制
发表时间：
2013-12-01
期刊：
影响因子：
3.5
通讯作者：
Bao, Dapeng
中科院分区：
文献类型：
--
作者：
Wu, Lin;van Peer, Arend;Bao, Dapeng
During the life cycle of heterothallic tetrapolar Agaricomycetes such as Lentinula edodes (Berk.) Pegler, the mating type system, composed of unlinked A and B loci, plays a vital role in controlling sexual development and resulting formation of the fruit body. L edodes is produced worldwide for consumption and medicinal purposes, and understanding its sexual development is therefore of great importance. A considerable amount of mating type factors has been indicated over the past decades but few genes have actually been identified, and no complete genetic structures of L. edodes B mating-type loci are available. In this study, we cloned the matB regions from two mating compatible L edodes strains, 939P26 and 939P42. Four pheromone receptors were identified on each new matB region, together with three and four pheromone precursor genes in the respective strains. Gene polymorphism, phylogenetic analysis and distribution of pheromone receptors and pheromone precursors clearly indicate a bipartite matB locus, each sublocus containing a pheromone receptor and one or two pheromone precursors. Detailed sequence comparisons of genetic structures between the matB regions of strains 939P42, 939P26 and a previously reported strain SUP2 further supported this model and allowed identification of the B mating type subloci borders. Mating studies confirmed the control of B mating by the identified pheromone receptors and pheromones in L. edodes. (C) 2013 Elsevier B.V. All rights reserved.
