Identification of common traits in improved xylose-growing Saccharomyces cerevisiae for inverse metabolic engineering
Identification of common traits in improved xylose-growing Saccharomyces cerevisiae for inverse metabolic engineering
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DOI：
10.1002/yea.1638
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发表时间：
2008-11-01
期刊：
影响因子：
2.6
通讯作者：
Gorwa-Grauslund, Mane-F.
中科院分区：
文献类型：
--
作者：
Bengtsson, Oskar;Jeppsson, Marie;Gorwa-Grauslund, Mane-F.
通过全基因组转录分析比较了4株木糖生长增强的重组酿酒酵母（TMB3400、C1、C5和BH 42）与2株对照菌株（TMB3399、TMB3001），以确定逆代谢工程的新靶点。选择在所有改良菌株中具有改变的表达水平的13个基因的子集用于进一步分析。构建了13个验证菌株和2个参考菌株，研究了这些基因在木糖利用型S.啤酒。在五种情况下观察到木糖的需氧生长速率提高。过表达SOL3和TAL1的菌株比其参考菌株生长快19%和24%，并且携带YLR 042C、MNI 1或RPA 49缺失的菌株比其参考菌株生长快173%、62%和90%。版权所有（C）2008约翰威利父子有限公司
Four recombinant Saccharomyces cerevisiae strains with enhanced xylose growth (TMB3400, C1, C5 and BH42) were compared with two control strains (TMB3399, TMB3001) through genome-wide transcription analysis in order to identify novel targets for inverse metabolic engineering. A subset of 13 genes with changed expression levels in all improved strains was selected for further analysis. Thirteen validation strains and two reference strains were constructed to investigate the effect of overexpressing or deleting these genes in xylose-utilizing S. cerevisiae. Improved aerobic growth rates on xylose were observed in five cases. The strains overexpressing SOL3 and TAL1 grew 19% and 24% faster than their reference strain, and the strains carrying deletions of YLR042C, MNI1 or RPA49 grew 173%, 62% and 90% faster than their reference strain. Copyright (C) 2008 John Wiley & Sons, Ltd.