Localized maternal proteins in Xenopus revealed by subtractive immunization
Localized maternal proteins in Xenopus revealed by subtractive immunization
复制标题
DOI：
10.1006/dbio.1997.8773
复制
发表时间：
1997-12-15
影响因子：
2.7
通讯作者：
Mowry, KL
中科院分区：
文献类型：
--
作者：
Denegre, JM;Ludwig, ER;Mowry, KL
人们很早就认识到，卵子中细胞质决定簇的定位可以为胚胎中的模式化提供基础。因此，早期卵裂球细胞命运的差异是受精前信息分子不对称分布的结果。青蛙卵在受精前存在一个不对称轴，即动物/植物轴，并且发育信息的定位似乎沿着该轴极化。此类发育信息可以定位为 RNA 或蛋白质；非洲爪蟾卵母细胞中的局部 RNA 已得到充分记录，其中一些被认为在轴向模式中发挥作用。虽然很明显并非所有局部母体成分都是 RNA，但对可能位于爪蟾卵内的母体蛋白知之甚少。在本研究中，我们采用了一种新方法来鉴定爪蟾卵内的局部母体蛋白。使用消减免疫策略，我们产生了单克隆抗体，可以识别仅限于受精卵植物皮层的抗原。对卵子发生过程中生物发生的分析揭示了两种不同的皮质定位模式。这些局部抗原中至少有三个是蛋白质，这些局部蛋白质可以代表在模式形成中发挥作用的母体决定因素。 (C) 1997 年学术出版社。
It has long been appreciated that the localization of cytoplasmic determinants in the egg can provide the foundation for patterning in the embryo. Differences in cell fate among the early blastomeres are thus a consequence of asymmetric distributions of informational molecules prior to fertilization. The frog egg has a single axis of asymmetry present prior to fertilization, the animal/vegetal axis, and the localization of developmental information appears to be polarized along this axis. Such developmental information can be localized as either RNA or protein; localized RNAs are well documented in the Xenopus oocyte, and some are thought to play roles in axial patterning. While it is apparent that not all of the localized maternal components are RNAs, much less is known about maternal proteins that might be localized in the In the present study, we have taken a novel approach to identify localized maternal proteins within the Xenopus egg. Using a subtractive immunization strategy, we have generated monoclonal antibodies which recognize antigens that are restricted to the vegetal cortex of fertilized eggs. Analysis of biogenesis during oogenesis reveals two distinct patterns of localization to the cortex. At least three of these localized antigens are proteins, and these localized proteins could represent maternal determinants with roles in patterning. (C) 1997 Academic Press.