ASYNCHRONOUS DNA-REPLICATION WITHIN THE HUMAN BETA-GLOBIN GENE LOCUS
ASYNCHRONOUS DNA-REPLICATION WITHIN THE HUMAN BETA-GLOBIN GENE LOCUS
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DOI：
10.1073/pnas.85.21.8081
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发表时间：
1988-11-01
影响因子：
11.1
通讯作者：
GROUDINE, M
中科院分区：
文献类型：
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作者：
EPNER, E;FORRESTER, WC;GROUDINE, M
人类β-珠蛋白基因座的DNA复制的时间已经通过来自S期不同阶段的细胞的新合成的BrdUrd取代的DNA的印迹杂交来研究。使用跨人类β-珠蛋白基因座跨>120kb的探针，我们表明该结构域的大部分复制了人类红白血病细胞系K562中的早期S期和淋巴细胞系Manca中的中晚期S期。然而，在 K562 细胞中，三个小区域显示出与相邻序列截然不同的复制模式。这些岛位于γ-球蛋白间基因区域，位于ε-球蛋白基因5”处约20 kilobases和β-球蛋白基因3” 处约20 kilobases，在后期和整个S期复制。 K562细胞的α-珠蛋白基因区域中也存在类似的区域。我们认为这些区域可能代表复制叉的终止位点。
The timing of DNA replication of the human .beta.-globin gene locus has been studied by blot hybridization of newly synthesized BrdUrd-substituted DNA from cells in different stages of the S phase. Using probes that span > 120 kilobases across the human .beta.-globin gene locus, we show that the majority of this domain replicates the early S phase in the human erythroleukemia cell line K562 and in middle-to-late S phase in the lymphoid cell line Manca. However, in K562 cells three small regions display a strikingly different replication pattern than adjacent sequences. These islands, located in the inter-.gamma.-globin gene region and approximately 20 kilobases 5'' to the .epsilon.-globin gene and 20 kilobases 3'' to the .beta.-globin gene, replicate later and throughout S phase. A similar area is also present in the .alpha.-globin gene region in K562 cells. We suggest that these regions may represent sites of termination of replication forks.