Long Noncoding RNA LINC00173 Promotes NUTF2 Expression Through Sponging miR-765 and Facilitates Tumorigenesis in Glioma
Long Noncoding RNA LINC00173 Promotes NUTF2 Expression Through Sponging miR-765 and Facilitates Tumorigenesis in Glioma
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DOI：
10.2147/cmar.s262279
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发表时间：
2020-01-01
影响因子：
3.3
通讯作者：
Wu, Jun
中科院分区：
文献类型：
--
作者：
Du, Qinghua;Liu, Jin;Wu, Jun
背景：胶质瘤是癌症相关死亡的主要原因之一。本研究旨在探讨长链非编码RNA（longnoncodingRNA，1 ncRNA）LINC 00173在胶质瘤发生发展中的作用及机制。生存率采用Kaplan Meier法分析。CCK 8、集落形成和EdU测定用于测量细胞增殖，而transwell用于测定细胞迁移和侵袭。结果：LINC 00173在胶质瘤组织和细胞中表达增强。LINC 00173高表达者预后差。LINC 00173的缺失抑制增殖、迁移和侵袭。LINC 00173与miR-765相互作用以增强NUTF 2表达。胶质瘤组织中miR-765的表达与LINC 00173和NUTF 2呈负相关。神经胶质瘤组织中NUTF 2水平升高。结论：LINC 00173通过靶向miR-765/NUTF 2轴促进胶质瘤的进展。
Background: Glioma is one of the leading causes of cancer-related deaths. This study aimed to investigate the function and mechanism of long noncoding RNA (1ncRNA) LINC00173 in the regulation of glioma progression.Methods: LINC00173 expression was measured using qRT-PCR. Survival rate was analyzed through Kaplan Meier method. CCK8, colony formation and EdU assays were performed to measure cell proliferation while transwell was used to determine cell migration and invasion. Luciferase reporter assay was conducted to test RNA interaction.Results: LINC00173 expression was elevated in glioma tissues and cells. LINC00173 high expression predicted poor prognosis. Loss of LINC00173 inhibited proliferation, migration and invasion. LINC00173 interacted with miR-765 to enhance NUTF2 expression. MiR-765 expression was negatively correlated with LINC00173 and NUTF2 in glioma tissues. NUTF2 level was increased in glioma tissues. NUTF2 overexpression rescued the potential of proliferation, migration and invasion in LINC00173-silenced cells.Conclusion: Our research demonstrated that LINC00173 promotes glioma progression through targeting miR-765/NUTF2 axis.