Relaxing constraints on data acquisition and position detection for trap stiffness calibration in optical tweezers
Relaxing constraints on data acquisition and position detection for trap stiffness calibration in optical tweezers
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DOI：
10.1364/oe.394632
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发表时间：
2020-05-25
期刊：
影响因子：
3.8
通讯作者：
Guerreiro, Thiago
中科院分区：
文献类型：
--
作者：
Melo, Bruno;Almeida, Felipe;Guerreiro, Thiago
光镊在从生物学到物理学的各个领域都有应用。使用捕获的粒子进行定量测量所需的基本步骤之一是校准镊子的弹簧常数。这可以通过功率谱密度分析，从粒子的位置的前向散射检测来完成。在这项工作中，我们提出并实验测试简化这样的测量过程，旨在减少后处理的记录数据和处理与采集设备，具有频率相关的电子噪声。在简化镊子设置的同一行中，我们还提出了一个刀口检测方案，可以替代标准的位置敏感探测器。(C)根据OSA开放获取出版协议的条款，2020年美国光学学会
Optical tweezers find applications in various fields, ranging from biology to physics. One of the fundamental steps necessary to perform quantitative measurements using trapped particles is the calibration of the tweezer's spring constant. This can be done through power spectral density analysis, from forward scattering detection of the particle's position. In this work we propose and experimentally test simplifications to such measurement procedure, aimed at reducing post-processing of recorded data and dealing with acquisition devices that have frequency-dependent electronic noise. In the same line of simplifying the tweezer setup we also present a knife-edge detection scheme that can substitute standard position sensitive detectors. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement