DIFFERENTIAL DISTRIBUTION OF N-ACETYLASPARTYLGLUTAMATE AND N-ACETYLASPARTATE IMMUNOREACTIVITIES IN RAT FOREBRAIN
DIFFERENTIAL DISTRIBUTION OF N-ACETYLASPARTYLGLUTAMATE AND N-ACETYLASPARTATE IMMUNOREACTIVITIES IN RAT FOREBRAIN
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DOI：
10.1007/bf01181604
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发表时间：
1995-06-01
期刊：
影响因子：
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通讯作者：
NAMBOODIRI, MAA
中科院分区：
文献类型：
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作者：
MOFFETT, JR;NAMBOODIRI, MAA
使用不同的碳二亚胺固定方案和抗体纯化方法，关于N-乙酰天冬氨酸在大鼠前脑中的定位，已有相互矛盾的免疫组织化学数据报道。在一个病例中，N-乙酰天冬氨酸谷氨酸免疫反应在所有皮质和等皮质区域的明显中间神经元中观察到，提示可能与GABA共存。在另一例中，在新皮质和海马区的许多锥体细胞中观察到强烈的免疫反应，提示与谷氨酸或天冬氨酸共存。协调这些不同的发现对于理解N-乙酰天冬氨酸谷氨酸在神经系统功能中的作用至关重要。分阶段纯化N-乙酰天冬氨酸和结构相关分子N-乙酰天冬氨酸的抗体，用固相免疫分析法检测抗体与N-乙酰天冬氨酸和N-乙酰天冬氨酸蛋白结合物的交叉反应。抗N-乙酰天冬氨酸抗体对N-乙酰天冬氨酸蛋白结合物的交叉反应性降低到约1%，消除了大鼠前脑中大多数锥体神经元的显着染色。利用高纯度的抗体，观察了N-乙酰天冬氨酸和N-乙酰天冬氨酸在大鼠端脑和间脑的几个主要区域的分布，发现它们是不同的。在特定的神经元群体中观察到N-乙酰天冬氨酸-谷氨酸免疫反应，包括许多被认为使用GABA作为神经递质的群体。其中包括苍白球、苍白球腹侧、中脑内侧核、丘脑网状核，以及散在分布于等皮质和分配皮质各层的非锥体神经元。N-乙酰天冬氨酸免疫反应在大鼠前脑中的分布比N-乙酰天冬氨酸免疫反应更广泛，在许多锥体神经元中表现最强。虽然N-乙酰天冬氨酸免疫反应在大多数神经元中存在，但在不同类型的神经元之间表现出很大的强度差异。
Contradictory immunohistochemical data have been reported on the localization of N-acetylaspartylglutamate in the rat forebrain, using different carbodiimide fixation protocols and antibody purification methods. In one case, N-acetylaspartylglutamate immunoreactivity was observed in apparent interneurons throughout all allocortical and isocortical regions, suggesting possible colocalization with GABA. In another case, strong immunoreactivity was observed in numerous pyramidal cells in neocortex and hippocampus, suggesting colocalization with glutamate or aspartate. Reconciling these disparate findings is crucial to understanding the role of N-acetylaspartylglutamate in nervous system function. Antibodies to N-acetylaspartylglutamate and a structurally related molecule, N-acetylaspartate, were purified in stages, and their crossreactivities with protein conjugates of N-acetylaspartylglutamate and N-acetylaspartate were monitored at each stage by solid-phase immunoassay. Reduction of the cross-reactivity of the anti-N-acetylaspartylglutamate antibodies to N-acetylaspartateprotein conjugates to about 1% eliminated significant staining of most pyramidal neurons in the rat forebrain. Utilizing highly purified antibodies, the distributions of N-acetylaspartylglutamate and N-acetylaspartate were examined in several major telencephalic and diencephalic regions of the rat, and were found to be distinct. N-acetylaspartylglutamate-immunoreactivity was observed in specific neuronal populations, including many groups thought to use GABA as a neurotransmitter. Among these were the globus pallidus, ventral pallidum, ent.opeducular nucleus, thalamic reticular nucleus, and scattered nonpyramidal neurons in all layers of isocortex and allocortex. N-acetylaspartate-immunoreactivity was more broadly distributed than N-acetylaspartylglutamate-immunoreactivity in the rat forebrain, appearing strongest in many pyramidal neurons. Although N-acetylaspartate-immunoreactivity was found in most neurons, it exhibited a great range of intensities between different neuronal types.