The Development and Characterization of Near-Isogenic and Pyramided Lines Carrying Resistance Genes to Brown Planthopper with the Genetic Background of Japonica Rice (Oryza sativa L.)
The Development and Characterization of Near-Isogenic and Pyramided Lines Carrying Resistance Genes to Brown Planthopper with the Genetic Background of Japonica Rice (Oryza sativa L.)
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DOI：
10.3390/plants8110498
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发表时间：
2019-11-01
期刊：
影响因子：
4.5
通讯作者：
Fujita, Daisuke
中科院分区：
文献类型：
--
作者：
Nguyen, Cuong D.;Verdeprado, Holden;Fujita, Daisuke
褐飞虱Nilaparvata lugens Stal.是亚洲水稻的主要害虫。寄主植物的抗病性对减轻稻飞虱对水稻的危害具有巨大的潜力。然而，根据BPH的毒力，抗性基因的有效性在空间和时间上是不同的。了解褐飞虱对抗性基因的毒力模式对于有效和可持续地部署抗性水稻品种是必要的。为了研究褐飞虱的毒力模式，选择了7个含有单一褐飞虱抗性基因的近等基因系（NIL），以粳稻台中65（T65）为遗传背景，培育了BPH 2-NIL、BPH 3-NIL、BPH 17-NIL、BPH 20-NIL、BPH 21-NIL、BPH 32-NIL和BPH 17-ptb-NIL等15个抗虫基因的抗虫品系，并评估了对两个BPH种群（分别于1966年和2013年在日本收集的Hadano-66和Koshi-2013）的抗性水平。许多NIL和PYL对Hadano-66群体具有抗性，但对Koshi-2013群体的有效性较低。在PYL中，BPH 20 + BPH 32-PYL和BPH 2 + BPH 3 + BPH 17-PYL对Koshi-2013具有相对高的抗褐飞虱性。在本研究中开发的近等基因系和潜在基因系将有助于在部署抗性水稻品种之前监测褐飞虱的毒性，并在区域性毒性水平增加的背景下提高水稻对褐飞虱的抗性。
The brown planthopper (BPH: Nilaparvata lugens Stal.) is a major pest of rice, Oryza sativa, in Asia. Host plant resistance has tremendous potential to reduce the damage caused to rice by the planthopper. However, the effectiveness of resistance genes varies spatially and temporally according to BPH virulence. Understanding patterns in BPH virulence against resistance genes is necessary to efficiently and sustainably deploy resistant rice varieties. To survey BPH virulence patterns, seven near-isogenic lines (NILs), each with a single BPH resistance gene (BPH2-NIL, BPH3-NIL, BPH17-NIL, BPH20-NIL, BPH21-NIL, BPH32-NIL and BPH17-ptb-NIL) and fifteen pyramided lines (PYLs) carrying multiple resistance genes were developed with the genetic background of the japonica rice variety, Taichung 65 (T65), and assessed for resistance levels against two BPH populations (Hadano-66 and Koshi-2013 collected in Japan in 1966 and 2013, respectively). Many of the NILs and PYLs were resistant against the Hadano-66 population but were less effective against the Koshi-2013 population. Among PYLs, BPH20+BPH32-PYL and BPH2+BPH3+BPH17-PYL granted relatively high BPH resistance against Koshi-2013. The NILs and PYLs developed in this research will be useful to monitor BPH virulence prior to deploying resistant rice varieties and improve rice's resistance to BPH in the context of regionally increasing levels of virulence.