INTERACTION OF TOBACCO MOSAIC-VIRUS PROTEIN WITH SYNTHETIC POLYNUCLEOTIDES CONTAINING A FLUORESCENT LABEL - OPTICAL-PROPERTIES OF POLY (A, EPSILON A) AND POLY (C, EPSILON C) COPOLYMERS AND ENERGY MIGRATION FROM TRYPTOPHAN TO 1,N6-ETHENOADENINE OR 3,N4-ETHENOCYTOSINE RESIDUES IN RNP
INTERACTION OF TOBACCO MOSAIC-VIRUS PROTEIN WITH SYNTHETIC POLYNUCLEOTIDES CONTAINING A FLUORESCENT LABEL - OPTICAL-PROPERTIES OF POLY (A, EPSILON A) AND POLY (C, EPSILON C) COPOLYMERS AND ENERGY MIGRATION FROM TRYPTOPHAN TO 1,N6-ETHENOADENINE OR 3,N4-ETHENOCYTOSINE RESIDUES IN RNP
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DOI：
10.1093/nar/5.11.4225
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发表时间：
1978-01-01
影响因子：
14.9
通讯作者：
BOGDANOV, AA
中科院分区：
文献类型：
--
作者：
LEDNEVA, RK;RAZJIVIN, AP;BOGDANOV, AA
本文提出了一种分光光度法测定聚（A，εA）和聚（C，εC）共聚物的改性度和摩尔消光系数的方法。研究了共聚物的吸收和荧光参数与改性程度的关系。改性残基在共聚物中的分布不同于无规分布，并取决于改性条件。研究了TMV蛋白与共聚物之间的相互作用。该蛋白与低或中等修饰程度的poly（A，εA）相互作用，对poly（εA）无活性。相反，将εC引入多核苷酸促进poly（C，εC）与TMV蛋白之间的复合物形成。对荧光发射和激发光谱的分析表明，在RNP中发生了从色氨酸到εA或εC的能量转移，并允许人们估计Trp（推测为Trp 52）与病毒中RNA碱基结合区之间的平均距离为17至20 nm。
A spectrophotometric method for determination of the modification degrees and molar extinction coefficients for poly(A, εA) and poly(C, εC) copolymers has been developed. Dependence of some absorption and fluorescence parameters of the copolymere on the modification degree has been studied. Distribution of modified residues in copolymers differs from random and depends on modification conditions. Interaction between the TMV protein and copolymers has been investigated. The protein interacts with poly(A, εA) of low or medium modifica tion degree and displays no activity with respect to poly(εA). On the contrary, introduction of εC to the polynucleotide promotes complex formation between poly(C, εC) and TMV protein. Analysis of the fluorescence emission and excitation spectra has revealed energy transfer from tryptophan to εA or εC in the RNP to occur and permits one to estimate the average distance between Trp (presumably Trp 52) the RNA base binding region in the virus to be 17 to 20 Å.