Nitrate transporter NPF7.3/NRT1.5 plays an essential role in regulating phosphate deficiency responses in Arabidopsis
Nitrate transporter NPF7.3/NRT1.5 plays an essential role in regulating phosphate deficiency responses in Arabidopsis
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硝酸盐转运蛋白 NPF7.3/NRT1.5 在调节拟南芥磷酸盐缺乏反应中发挥重要作用
DOI：
10.1016/j.bbrc.2018.11.118
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发表时间：
2019-01-01
影响因子：
3.1
通讯作者：
Ma, Qing
中科院分区：
文献类型：
--
作者：
Cui, Yan-Nong;Li, Xiao-Ting;Ma, Qing
AtNPF7.3/AtNRT1.5是一种硝酸盐转运蛋白，驱动NO3-从根到地上部的运输，也通过影响根的发育和K+的运输参与调节拟南芥对K+剥夺的反应。然而，NPF7.3/NRT1.5是否在调节植物对其他营养素缺乏的反应中起作用仍不清楚。在这项研究中，我们发现AtNPF7.3/AtNRT1.5的表达在根中占主导地位，并且在很大程度上受到磷酸盐（Pi）饥饿的诱导。atnrt 1.5突变体表现出显着较长的主根沿着与显着减少侧根密度在缺磷条件下比野生型植物，和这些形态差异的根被消除在一定程度上由乙烯合成拮抗剂Co 2+。进一步的分析表明，重要的磷饥饿诱导的基因，这是直接参与磷的运输，动员和分配，显着高于在野生型植物在磷饥饿条件下的atnrt1.5突变体的表达，因此，atnrt1.5突变体保留较高的组织磷浓度。综上所述，我们的研究结果表明，NPF7.3/NRT1.5是一个重要的组成部分，在拟南芥中的磷酸盐缺乏反应的调节。(C)2018爱思唯尔公司All rights reserved.
AtNPF7.3/AtNRT1.5, which is a nitrate transporter that drives root-to-shoot transport of NO3-, is also involved in modulating the response to K+ deprivation in Arabidopsis by affecting root development and K+ transport. However, whether NPF7.3/NRT1.5 functions in regulating plant responses to deficiencies of other nutrients remains unknown. In this study, we found that the expression of AtNPF7.3/AtNRT1.5 was predominant in the roots and was substantially induced by phosphate (Pi) starvation. The atnrt1.5 mutants displayed conspicuously longer primary roots along with a significantly reduced lateral root density under Pi-deficient conditions than did the wild-type plants, and these morphological differences in the roots were eliminated to a certain extent by the ethylene synthesis antagonist Co2+. Further analyses revealed that the expression of important Pi starvation-induced genes, which are directly involved in Pi transport, mobilization and distribution, were significantly higher in the atnrt1.5 mutants than that in the wild-type plants under Pi-starvation conditions; therefore, the atnrt1.5 mutants retained higher tissue Pi concentrations. Taken together, our results suggest that NPF7.3/NRT1.5 is an important component in the regulation of phosphate deficiency responses in Arabidopsis. (C) 2018 Elsevier Inc. All rights reserved.