Microtubules orient the mitotic spindle in yeast through dynein-dependent interactions with the cell cortex.
Microtubules orient the mitotic spindle in yeast through dynein-dependent interactions with the cell cortex.
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DOI：
10.1083/jcb.138.3.629
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发表时间：
1997-08-11
期刊：
影响因子：
--
通讯作者：
Stearns T
中科院分区：
文献类型：
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作者：
Carminati JL;Stearns T
有丝分裂纺锤体的正确定向对芽殖酵母细胞成功分裂至关重要。为了研究纺锤体定向的机制，我们使用绿色荧光蛋白（GFP）-微管蛋白融合蛋白观察活酵母细胞中的微管。GFP-微管蛋白被掺入微管中，允许细胞质和纺锤体微管的可视化，并且不干扰正常的微管功能。酵母细胞中的微管表现出动态不稳定性，尽管它们的生长和收缩比动物细胞中的微管慢。酵母微管的动力学特性在细胞周期中受到调节。细胞质微管的行为揭示了不同的相互作用与细胞皮质，导致相关的纺锤体运动和方向。动力蛋白突变细胞在这些皮质相互作用中存在缺陷，导致纺锤体方向错误。此外，微管动力学改变动力蛋白的情况下。这些结果表明，微管和动力蛋白相互作用，产生动态的皮质相互作用，这些相互作用的结果，在力驱动主轴方向。
Proper orientation of the mitotic spindle is critical for successful cell division in budding yeast. To investigate the mechanism of spindle orientation, we used a green fluorescent protein (GFP)–tubulin fusion protein to observe microtubules in living yeast cells. GFP–tubulin is incorporated into microtubules, allowing visualization of both cytoplasmic and spindle microtubules, and does not interfere with normal microtubule function. Microtubules in yeast cells exhibit dynamic instability, although they grow and shrink more slowly than microtubules in animal cells. The dynamic properties of yeast microtubules are modulated during the cell cycle. The behavior of cytoplasmic microtubules revealed distinct interactions with the cell cortex that result in associated spindle movement and orientation. Dynein-mutant cells had defects in these cortical interactions, resulting in misoriented spindles. In addition, microtubule dynamics were altered in the absence of dynein. These results indicate that microtubules and dynein interact to produce dynamic cortical interactions, and that these interactions result in the force driving spindle orientation.