A 384-Well Microtiter Plate Model for Candida Biofilm Formation and Its Application to High-Throughput Screening.
A 384-Well Microtiter Plate Model for Candida Biofilm Formation and Its Application to High-Throughput Screening.
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DOI：
10.1007/978-1-0716-3155-3_5
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发表时间：
2023-01-01
期刊：
影响因子：
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通讯作者：
Lopez-Ribot, Jose L
中科院分区：
文献类型：
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作者：
Ajetunmobi, Olabayo H;Wall, Gina;Lopez-Ribot, Jose L
念珠菌病，念珠菌属引起的感染，是最常见的医院感染之一，使越来越多的患者受到感染。抗真菌治疗选择很少，疗效有限。此外，生物膜形成经常与念珠菌病的不同表现相关，并进一步使治疗复杂化。因此，迫切需要新的有效治疗剂，特别是具有抗生物膜活性的那些。在这里，我们描述了一种新的，简单的，快速的，经济的，高度可重复的384孔微量滴定板模型的发展，白色念珠菌和耳念珠菌生物膜的形成及其在高通量筛选（HTS）技术中的应用。
Candidiasis, infections caused by Candida spp., represents one of the most common nosocomial infections afflicting an expanding number of compromised patients. Antifungal therapeutic options are few and show limited efficacy. Moreover, biofilm formation is frequently associated with different manifestations of candidiasis and further complicates therapy. Thus, there is an urgent need for new effective therapeutic agents, particularly those with anti-biofilm activity. Here we describe the development of a novel, simple, fast, economical, and highly reproducible 384-well microtiter plate model for the formation of both Candida albicans and Candida auris biofilms and its application in high-throughput screening (HTS) techniques.