'Candidatus phytoplasma japonicum', a new phytoplasma taxon associated with Japanese Hydrangea phyllody.
'Candidatus phytoplasma japonicum', a new phytoplasma taxon associated with Japanese Hydrangea phyllody.
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“Candidatus phytoplasma japonicum”，一个与日本绣球叶状相关的新植原体分类群。
DOI：
10.1099/00207713-49-3-1275
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
S. Namba
中科院分区：
文献类型：
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作者：
T. Sawayanagi;N. Horikoshi;Tsutomu Kanehira;Masayuki Shinohara;Assunta Bertaccini;M.;Chuji Hiruki;S. Namba
在田间种植的绣球花（hortensia）患病标本中发现了一种植物原体，表现出典型的层状学症状。利用植原体特异性引物进行DNA PCR扩增，检测出所有病株的植原体DNA。在健康的霍兰幼苗中未发现植原体DNA。扩增的16S rDNA的RFLP模式不同于其他植物源，包括6株外源植物源。基于RFLP，将日本绣球植物原体（Japanese Hydrangea phyllody， JHP）分类为植物原体16S rRNA组I （aster yellow, onion yellow，所有已报道的hortensia phytoplasmas及其相关植物原体）中一个新亚群的代表。通过对该植物和其他I类植物原体16S rRNA基因序列的系统发育分析，JHP植物原体在Mollicutes类植物原体分支中属于一个独特的亚群（亚群Id）。由16S rRNA基因序列构建的系统发育树与JHP植物原体与其最近的亲缘植物澳洲葡萄黄（AUSGY）、Phormium yellow leaf （PYL）、Capsicum annuum Stolbur （STOL）和葡萄Vergilbungskrankheit （VK）具有共同祖先的假设一致。JHP植物原体DNA的独特性质清楚地表明它代表了一个新的分类单元，“Candidatus phytoplasma japonicum”。
A phytoplasma was discovered in diseased specimens of field-grown hortensia (Hydrangea spp.) exhibiting typical phyllody symptoms. PCR amplification of DNA using phytoplasma specific primers detected phytoplasma DNA in all of the diseased plants examined. No phytoplasma DNA was found in healthy hortensia seedlings. RFLP patterns of amplified 16S rDNA differed from the patterns previously described for other phytoplasmas including six isolates of foreign hortensia phytoplasmas. Based on the RFLP, the Japanese Hydrangea phyllody (JHP) phytoplasma was classified as a representative of a new subgroup in the phytoplasma 16S rRNA group I (aster yellows, onion yellows, all of the previously reported hortensia phytoplasmas, and related phytoplasmas). A phylogenetic analysis of 16S rRNA gene sequences from this and other group I phytoplasmas identified the JHP phytoplasma as a member of a distinct sub-group (sub-group Id) in the phytoplasma clade of the class Mollicutes. The phylogenetic tree constructed from 16S rRNA gene sequences was consistent with the hypothesis that the JHP phytoplasma and its closest known relatives, the Australian grapevine yellows (AUSGY), Phormium yellow leaf (PYL), Stolbur of Capsicum annuum (STOL) and Vergilbungskrankheit of grapevine (VK) share a common ancestor. The unique properties of the DNA from the JHP phytoplasma clearly establish that it represents a new taxon, 'Candidatus Phytoplasma japonicum'.