Effects of anesthetics on systemic hemodynamics in mice
Effects of anesthetics on systemic hemodynamics in mice
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DOI：
10.1152/ajpheart.01192.2003
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发表时间：
2004-10-01
影响因子：
4.8
通讯作者：
Smith, TL
中科院分区：
文献类型：
--
作者：
Janssen, BJA;De Celle, T;Smith, TL
本研究的目的是比较四种常用麻醉方案对小鼠全身血流动力学的影响，这些小鼠长期使用仪器直接和连续测量心输出量（CO）。小鼠（CD-1、Swiss和C57BL6品系）在升主动脉周围放置瞬时血流探针测量CO。4 ~ 5天后将动脉导管插入主动脉测量血压。完全恢复后，在清醒状态下测量动物的血流动力学参数，包括搏气量、心率、CO、平均动脉压（MAP）和总外周阻力。然后用异氟醚（Iso）、聚氨酯、戊巴比妥钠或氯胺酮-噻嗪（K-X）诱导小鼠全身麻醉。这些药物的剂量和给药途径是根据这些动物的一般外科手术的需要给予的。与清醒静息状态下的动物相比，MAP和CO在所有麻醉干预期间均有所下降，其中Iso的血流动力学影响最小（MAP, - 24 +/- 3%； CO, - 5 +/- 7%; n = 15只小鼠），K-X的血流动力学影响最大（MAP, - 51 +/- 6%； CO, - 37 +/- 9%; n = 8只小鼠）。K-X的血流动力学作用可通过α(2)受体拮抗剂阿替帕唑完全拮抗（n = 8只小鼠）。这些结果表明，与非挥发性麻醉剂相比，麻醉剂Iso对小鼠全身血流动力学的影响更小。
The aim of this study was to compare the systemic hemodynamic effects of four commonly used anesthetic regimens in mice that were chronically instrumented for direct and continuous measurements of cardiac output ( CO). Mice (CD-1, Swiss, and C57BL6 strains) were instrumented with a transittime flow probe placed around the ascending aorta for CO measurement. An arterial catheter was inserted into the aorta 4 or 5 days later for blood pressure measurements. After full recovery, hemodynamic parameters including stroke volume, heart rate, CO, mean arterial pressure ( MAP), and total peripheral resistance were measured with animals in the conscious state. General anesthesia was then induced in these mice using isoflurane (Iso), urethane, pentobarbital sodium, or ketamine-xylazine (K-X). The doses and routes of administration of these agents were given as required for general surgical procedures in these animals. Compared with the values obtained for animals in the conscious resting state, MAP and CO decreased during all anesthetic interventions, and hemodynamic effects were smallest for Iso ( MAP, - 24 +/- 3%; CO, - 5 +/- 7%; n = 15 mice) and greatest for K-X (MAP, - 51 +/- 6%; CO, - 37 +/- 9%; n = 8 mice), respectively. The hemodynamic effects of K-X were fully antagonized by administration of the alpha(2)-receptor antagonist atipamezole ( n = 8 mice). These results indicate that the anesthetic Iso has fewer systemic hemodynamic effects in mice than the nonvolatile anesthetics.