Titin is an extraordinarily long, flexible, and slender myofibrillar protein.
Titin is an extraordinarily long, flexible, and slender myofibrillar protein.
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肌联蛋白是一种非常长、柔韧且纤细的肌原纤维蛋白。
DOI：
10.1073/pnas.81.12.3685
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发表时间：
1984
影响因子：
11.1
通讯作者：
Palter,D
中科院分区：
文献类型：
--
作者：
Wang,K;Ramirez-Mitchell,R;Palter,D
“肌联蛋白“是用于描述一对密切相关的巨道尔顿多肽的术语，所述巨道尔顿多肽一起是广泛的横纹肌中第三丰富的肌原纤维蛋白。有人提出肌联蛋白和另一种巨大的蛋白质，星云蛋白，是肌节内弹性细胞骨架晶格的主要成分。我们现在已经通过用Guba-Straub溶液提取，然后通过色谱法纯化了天然形式的肌联蛋白双联体的主导带，肌联蛋白-2（T2）。低角度阴影和负染色标本的电子显微镜显示，T2链自组装成极长（从0.1微米到1.0微米以上），灵活，可延伸的细长股（直径4-5纳米）与轴向周期性。此外，这些链倾向于联合形成丝状束和网状结构。因此，肌联蛋白似乎是理想的适合作为一个组成部分的弹性晶格，作为一个组织的支架或模板厚和薄的细丝。
" Titin " is a term used to describe a pair of closely related megadalton polypeptides that together are the third most abundant myofibrillar protein in a wide range of striated muscles. It has been proposed that titin and another giant protein, nebulin , are the major components of an elastic cytoskeletal lattice within the sarcomere. We have now purified the leading band, titin -2 (T2), of the titin doublet in native forms by extraction with Guba -Straub solution followed by chromatography. Electron microscopy of low-angle-shadowed and negatively stained specimens revealed that T2 chains self-assembled into extremely long (from 0.1 micron to over 1.0 micron), flexible, and extensible slender strands (4-5 nm in diameter) with axial periodicity. Furthermore, these strands tended to associate to form filamentous bundles and meshworks. Thus, titin appears to be ideally suited as a component of an elastic lattice that serves as an organizing scaffold or template for thick and thin filaments.