Sequential activation of three myogenic regulatory genes during somite morphogenesis in quail embryos.
Sequential activation of three myogenic regulatory genes during somite morphogenesis in quail embryos.
复制标题
鹌鹑胚胎体节形态发生过程中三个生肌调节基因的顺序激活。
DOI：
10.1016/0012-1606(92)90214-2
复制
发表时间：
1992
影响因子：
2.7
通讯作者：
EmersonJr,CP
中科院分区：
文献类型：
--
作者：
Pownall,ME;EmersonJr,CP
我们克隆了两个新的鹌鹑肌源性cDNA，即鹌鹑肌源性因子2（quailmyogenicfactor 2，qmf 2）和qmf 3，它们编码与哺乳动物肌源性因子myogenin和myf-5同源的螺旋-环-螺旋蛋白。这些研究表明，qmf 1和qmf 3被激活顺序在中间本地化的体节细胞，立即体节形成后，但之前的肌节形成。qmf 1，qmf 2，和qmf 3在肌节中表达的分隔体节。这些结果表明，鹌鹑体节中肌源性细胞谱系的确定是一个渐进的过程，受神经管对新形成体节中qmf调节基因表达的影响。
We report the cloning of two new quail myogenic cDNAs, quail myogenic factor 2 (qmf2) and qmf3, which encode helix-loop-helix proteins homologous to mammalian myogenic factors myogenin and myf-5.In situhybridization has been used to investigate the developmental expression ofqmf2andqmf3, as well asqmf1, the quail homologue to mammalianMyoD1, during the formation of the brachial somites. These studies show thatqmf1andqmf3are activated sequentially in medially localized somite cells, immediately following somite formation but prior to myotome formation.qmf1, qmf2, andqmf3are expressed in the myotome of compartmentalized somites. These findings suggest that determination of the myogenic cell lineage in quail somites is a progressive process controlled by influences of the neural tube on the expression of theqmfregulatory genes in newly forming somites.