Increased elastase secretion by peripheral blood monocytes in cystic fibrosis patients.
Increased elastase secretion by peripheral blood monocytes in cystic fibrosis patients.
复制标题
囊性纤维化患者外周血单核细胞弹性蛋白酶分泌增加。
DOI：
10.1111/j.1365-2249.1990.tb03290.x
复制
发表时间：
1990
影响因子：
4.6
通讯作者：
Rossen,RD
中科院分区：
文献类型：
--
作者：
Jones,MM;Seilheimer,DK;Pier,GB;Rossen,RD
囊性纤维化（CF）的发病率和死亡率主要是由于肺组织的破坏。宿主免疫反应可能部分地在这些患者的肺部破坏中发挥致病作用。为了进一步了解 CF 中的宿主免疫反应，我们检查了 CF 中外周血单核细胞的激活状态。 CF 单核细胞中的基线弹性蛋白酶活性比对照高 2.2 倍。铜绿假单胞菌类胞外多糖 (MEP) 和高分子量多糖 (HMP) 增加对照和 CF 单核细胞中的弹性蛋白酶活性，其中 CF 单核细胞的绝对增加更大。 CF 和对照单核细胞之间的基线或 MEP 刺激的白细胞介素 1 (IL-1) 或白细胞介素 6 (IL-6) 分泌没有差异。布洛芬增强了 MEP 和 HMP 刺激的弹性蛋白酶活性，而地塞米松在 CF 和对照单核细胞中抑制基线和刺激的弹性蛋白酶活性 > 20%。这些结果表明，CF 中的循环单核细胞在体内受到刺激，导致体外弹性蛋白酶活性显着升高。当外周血单核细胞响应趋化刺激而进入肺部时，弹性蛋白酶释放升高可能导致 CF 中的肺破坏。
Morbidity and mortality in cystic fibrosis (CF) is predominantly due to destruction of pulmonary tissue. The host immune response may, in part, play a pathogenic role in pulmonary destruction in these patients. To further understand host immune response in CF, we examined the state of activation of peripheral blood monocyles in CF. Baseline elastase activity was 2.2‐fold greater in the CF monocytes than in controls.Pseudomonas aeruginosamucoid exopolysaccharide (MEP) and high molecular weight polysaccharide (HMP) increased elastase activity in both control and CF monocytes, with a greater absolute increase in the CF monocytes. There was no difference in baseline or MEP‐stimulated secretion of interleukin‐1 (IL‐1) or interleukin‐6 (IL‐6) between CF and control monocytes. Ibuprofen enhanced both MEP and HMP‐stimulated elastase activity, whereas dexamethasone suppressed both baseline and stimulated elastase activity > 20% in both CF and control monocytes. These results suggest that circulating monocytes in CF are stimulatedin vivo, resulting in a remarkably elevated elastase activityin vitro. Elevated elastase release by peripheral blood monocytes as they enter the lung in response to chemotactic stimuli may contribute to lung destruction in CF.