Multivariate surface analysis of plasma-deposited tetraglyme for reduction of protein adsorption and monocyte adhesion
Multivariate surface analysis of plasma-deposited tetraglyme for reduction of protein adsorption and monocyte adhesion
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DOI：
10.1021/la0259297
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发表时间：
2003-03-04
期刊：
影响因子：
3.9
通讯作者：
Horbett, TA
中科院分区：
文献类型：
--
作者：
Shen, MC;Wagner, MS;Horbett, TA
植入的生物医疗设备上吸附的蛋白质介导了血小板和白细胞的黏附。射频等离子体沉积的四甘醇(CH3O(CH2CH2O)(4)CH3)在体外形成了一层类似PEO的涂层，可抵抗蛋白质吸附和单核细胞黏附。用不同的等离子体沉积功率(5-80W)制备了一系列表面化学成分不同的等离子体沉积的四乙醇胺表面，用化学分析电子能谱(ESCA)和飞行时间二次离子质谱仪(ToF-SIMS)进行了测量。在高功率等离子体作用下，纤维蛋白原和免疫球蛋白的吸附均增强。单核细胞黏附率与吸附蛋白的量呈线性相关。采用偏最小二乘回归的多元分析方法，对影响等离子体沉积四甘醇无污染性能的表面化学特征进行了研究。基于放置在等离子体反应器下游的沉积的四甘醇样品的偏最小二乘校正模型成功地预测了放置在反应器中间的沉积的四甘醇样品对纤维蛋白原的吸附。该模型确定了ESCA和ToF-SIMS的每个表面光谱变量如何对蛋白质吸附做出贡献。ESCA和TOF-SIMS测量表明，在纤维蛋白原吸附极低(200 ng/cm(2))的表面上，m/z 59和103处的碳在乙醚碳键中的比例较高。本研究阐明了沉积的四甘醇对蛋白质和细胞摄取的阻力最大的表面化学特征，并为工程改善无污染生物材料表面提供了指导。
Adsorbed proteins on implanted biomedical devices mediate platelet and leukocyte adhesion. Radio frequency plasma deposited tetraglyme (CH3O(CH2CH2O)(4)CH3), which forms a PEO-like coating, has been shown to resist protein adsorption and monocyte adhesion in vitro. By using different plasma deposition powers (5-80 W), we produced a series of plasma-deposited tetraglyme surfaces that varied in surface chemistry as measured by electron spectroscopy for chemical analysis (ESCA) and time-of-flight secondary ion mass spectrometry (ToF-SIMS). Both fibrinogen and IgG adsorption were increased on surfaces made at high plasma power. Monocyte adhesion correlated linearly with the amount of adsorbed protein. To identify the surface chemical features that contributed to the nonfouling properties of plasma-deposited tetraglyme, multivariate analysis using partial least squares (PLS) regression was applied. A PLS calibration model based on deposited tetraglyme samples placed downstream in the plasma reactor successfully predicted fibrinogen adsorption to deposited tetraglyme samples placed midstream in the reactor. The model identified how each surface spectral variable from ESCA and ToF-SIMS contributed to protein adsorption. The fraction of carbon in ether carbon linkages as measured by ESCA and ToF-SIMS peaks at m/z 59 and 103 was higher on surfaces that exhibited ultralow fibrinogen adsorption (200 ng/cm(2)). This study elucidated the surface chemical features of deposited tetraglyme that most affect its resistance to protein and cell uptake and provided guidelines for engineering improved nonfouling biomaterial surfaces.