Effects of surface finishing conditions on the biocompatibility of a nickel-chromium dental casting alloy
Effects of surface finishing conditions on the biocompatibility of a nickel-chromium dental casting alloy
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DOI：
10.1016/j.dental.2011.03.004
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发表时间：
2011-07-01
期刊：
影响因子：
5
通讯作者：
Fleming, Garry J. P.
中科院分区：
文献类型：
--
作者：
McGinley, Emma Louise;Coleman, David C.;Fleming, Garry J. P.
目标。评估表面处理条件（抛光或氧化铝颗粒空气研磨）对直接和间接暴露于镍铬（Ni-Cr） d. Sign (R) 10牙科铸造合金对口腔角化细胞生物相容性的影响。通过评估细胞活力和形态、代谢活性、细胞毒性和炎症细胞因子标志物的存在来评估生物相容性。铸造d. Sign (R) 10的圆盘，对氧化铝颗粒进行空气研磨，抛光一半，然后用轮廓术测定表面粗糙度。通过将椎间盘直接或间接（用浸泡液）与TR146单层接触来评估生物相容性。用ICP-MS测定金属离子释放量。台盼蓝染色法测定细胞活力，XTT法测定代谢活性，LDH法测定细胞毒性。采用夹心elisa法分析炎症细胞因子。抛光后的平均Ra值与氧化铝颗粒空气磨光片相比显著降低（P < 0.001），但金属离子释放量显著增加。与氧化铝颗粒空气研磨圆盘相比，抛光后的细胞密度显著降低，观察到直接或间接暴露。与氧化铝颗粒空气研磨圆盘相比，24 h时抛光圆盘的代谢活性显著降低，细胞毒性增加，炎症细胞因子标记物增加。研究镍铬牙科合金的精加工状况具有重要的临床意义。结合ICP-MS，利用细胞密度和形态、代谢活性、细胞毒性水平和炎症标志物对TR146上皮细胞的反应，使作者对牙科合金在口腔环境中经历的复杂过程有了更深入的了解。(C) 2011年牙科材料学会。Elsevier Ltd.出版。版权所有。
Objectives. To assess the effects of surface finishing condition (polished or alumina particle air abraded) on the biocompatibility of direct and indirect exposure to a nickel-chromium (Ni-Cr) d. Sign (R) 10 dental casting alloy on oral keratinocytes. Biocompatibility was performed by assessing cellular viability and morphology, metabolic activity, cellular toxicity and presence of inflammatory cytokine markers.Methods. Discs of d. Sign (R) 10 were cast, alumina particle air abraded and half were polished before surface roughness was determined by profilometry. Biocompatibility was assessed by placing the discs directly or indirectly (with immersion solutions) into contact with TR146 monolayers. Metal ion release was determined by ICP-MS. Cell viability was assessed by trypan blue dye exclusion, metabolic activity by XTT and cellular toxicity by LDH. Inflammatory cytokine analysis was performed using sandwich ELISAs.Results. The mean polished Ra value was significantly reduced (P < 0.001) compared with the alumina particle air abraded discs but metal ion release was significantly increased for the polished discs. Significant reductions in cell density of polished compared with alumina particle air abraded discs was observed following direct or indirect exposure. A significant reduction in metabolic activity, increase in cellular toxicity and an increase in the presence of inflammatory cytokine markers was highlighted for the polished relative to the alumina particle air abraded discs at 24 h.Significance. Finishing condition of the Ni-Cr dental alloy investigated has important clinical implications. The approach of employing cell density and morphology, metabolic activity, cellular toxicity levels and inflammatory marker responses to TR146 epithelial cells combined with ICP-MS afforded the authors an increased insight into the complex processes dental alloys undergo in the oral environment. (C) 2011 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.