Preparation and characterization of a novel rice plant-specific kinesin.
Preparation and characterization of a novel rice plant-specific kinesin.
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DOI：
10.1093/jb/mvj074
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发表时间：
2006-04
影响因子：
2.7
通讯作者：
N. Umeki;T. Mitsui;Nozomi Umezu;K. Kondo;S. Maruta
中科院分区：
文献类型：
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作者：
N. Umeki;T. Mitsui;Nozomi Umezu;K. Kondo;S. Maruta
驱动蛋白是一种ATP驱动的运动蛋白，在细胞内转运、有丝分裂和减数分裂、微管动力学控制和信号转导中发挥重要的生理作用。驱动蛋白家族分为亚家族。来源于脊椎动物的驱动蛋白种类已经被很好地表征。相比之下，植物驱动蛋白尚未得到充分的表征。在这项研究中，我们表达了一种新的水稻植物特异性驱动蛋白，K16，在大肠杆菌中的马达结构域，然后确定其酶的特性，并比较他们与驱动蛋白1。我们的研究结果表明，水稻驱动蛋白运动域具有不同的酶的特性，从那些众所周知的驱动蛋白1。
Kinesin is an ATP-driven motor protein that plays important physiological roles in intracellular transport, mitosis and meiosis, control of microtubule dynamics, and signal transduction. The kinesin family is classified into subfamilies. Kinesin species derived from vertebrates have been well characterized. In contrast, plant kinesins have yet to be adequately characterized. In this study, we expressed the motor domain of a novel rice plant-specific kinesin, K16, in Escherichia coli, and then determined its enzymatic characteristics and compared them with those of kinesin 1. Our findings demonstrated that the rice kinesin motor domain has different enzymatic properties from those of well known kinesin 1.