Hypotonic solution increases the slowly activating potassium current IsK expressed in xenopus oocytes.
Hypotonic solution increases the slowly activating potassium current IsK expressed in xenopus oocytes.
复制标题
低渗溶液增加爪蟾卵母细胞中表达的缓慢激活的钾电流IsK。
DOI：
10.1016/0006-291x(92)90661-4
复制
发表时间：
1992
影响因子：
3.1
通讯作者：
North,RA
中科院分区：
文献类型：
--
作者：
Busch,AE;Varnum,M;Adelman,JP;North,RA
用大鼠肾cDNA克隆转录的RNA在爪蟾卵母细胞中表达慢激活钾电流。低渗溶液（160 mOsmol/l；对照组为220 mOsmol/l）通过增加激活速率和减少激活电流所需的去极化来增加电流。在无钙溶液中没有观察到这种低压性作用，但不受斯陶孢素或钙调素拮抗剂W7的影响。细胞松弛素D降低了电流，阻止了低渗溶液的增加。结果表明，低渗溶液钾电流的增加可能是由于钙的进入和肌动蛋白网络的改变。
A slowly activating potassium current was expressed in Xenopus oocytes by injection of RNA transcribed from a rat kidney cDNA clone. Hypotonic solutions (160 mOsmol/l; control was 220 mOsmol/l) increased the current by increasing the rate of activation and by decreasing the depolarization needed to activate the current. This effect of hypotonicity was not observed in calcium-free solution, but was unaffected by staurosporine or the calmodulin antagonist W7. Cytochalasin D reduced the current and prevented the increase by hypotonic solution. The results suggest that the increase in this potassium current by hypotonic solution might result from calcium entry and changes in the actin network.