Microfaunal indicators, Ciliophora phylogeny and protozoan population shifts in an intermittently aerated and fed bioreactor.
Microfaunal indicators, Ciliophora phylogeny and protozoan population shifts in an intermittently aerated and fed bioreactor.
复制标题
间歇性曝气和补料生物反应器中的微型动物指标、纤毛类系统发育和原生动物种群变化。
DOI：
10.1016/j.jhazmat.2010.12.099
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发表时间：
2011
影响因子：
13.6
通讯作者：
P. Melidis
中科院分区：
文献类型：
--
作者：
S. Ntougias;Spartakos Tanasidis;P. Melidis
在配备间歇式曝气和进料系统的小型生物反应器的混合液中检查了微型动物群落结构。反应器在每 1 小时间歇通气 25 分钟和不同的进料条件（0.264、0.403 和 0.773kg BOD5/m3d）下运行。通过显微镜检查共鉴定出14个原生动物和后生动物类群。在0.403kg BOD5/m3d有机负荷条件下，固着纤毛虫是主要的原生动物类群，其次是爬行纤毛虫，而在0.773kg BOD5/m3d有机负荷条件下，固着纤毛虫种群数量显着增加。进行主成分分析和皮尔逊相关系数测试，以揭示微型动物群落与运行参数之间的关系。构建纤毛虫特异性18S rRNA基因克隆文库，以鉴定0.773kg BOD5/m3d有机负荷条件下的纤毛虫多样性。纤毛虫多样性包括 Aspidiscidae、Epistylidae、Opisthonectidae 和 Vorticellidae 的成员，其中大多数克隆与 Vorticella fusca 物种相关。纤毛纲之间至少存在一种新的系统发育联系。对纤毛虫群落进行分子表征和显微镜检查后进行的比较表明，对广泛纤毛虫类群的估计有助于生态考虑和废水处理厂运行条件的评估。
Microfauna community structure was examined in the mixed liquor of a bench-scale bioreactor equipped with an intermittent aeration and feeding system. The reactor was operated under an intermittent aeration of 25min in every 1h and varying feeding conditions (0.264, 0.403 and 0.773kg BOD5/m3d). A total of 14 protozoan and metazoan taxa were identified by microscopic examination. Sessile ciliates, followed by crawling ciliates, were the major protozoan groups under 0.403kg BOD5/m3d organic loading conditions, while sessile ciliate population was remarkably increased under an organic loading of 0.773kg BOD5/m3d. Principal Component Analysis and Pearson correlation coefficient tests were performed in order to reveal relationships between microfauna community and operational parameters. Ciliophora specific-18S rRNA gene clone library was constructed to identify ciliate diversity under 0.773kg BOD5/m3d organic loading conditions. Ciliophora diversity consisted of members of Aspidiscidae, Epistylidae, Opisthonectidae and Vorticellidae, with the majority of the clones being associated with the species Vorticella fusca. At least one novel phylogenetic linkage among Ciliophora was identified. Comparisons made after molecular characterization and microscopic examination of Ciliophora community showed that the estimation of broad ciliate groups is useful for ecological considerations and evaluation of the operational conditions in wastewater treatment plants.