Isothermal reactions for the amplification of oligonucleotides
Isothermal reactions for the amplification of oligonucleotides
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DOI：
10.1073/pnas.0730811100
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发表时间：
2003-04-15
影响因子：
11.1
通讯作者：
Galas, DJ
中科院分区：
文献类型：
--
作者：
Van Ness, J;Van Ness, LK;Galas, DJ
我们设计了一类用于扩增DNA的等温反应。这些均相反应快速合成由扩增模板的序列指定的短寡核苷酸(8-16个碱基)。不同版本的反应可以以线性或指数放大模式进行。这两个反应都需要简单、恒定的条件，放大的速度完全取决于控制反应中分子相互作用的分子参数。该反应的指数版本是一种分子链式反应，它使用每个线性反应的寡核苷酸产物来产生更多相同的寡核苷酸的生产者。这是一种高度敏感的连锁反应，可以由给定的DNA序列特异性地触发，并可以实现>；10(6)倍的扩增。这里描述了这一类中的几个类似的反应。指数反应的稳定性、速度和灵敏度表明，它将有助于快速检测样品中少量特定DNA序列的存在，以及其他一系列应用，包括目前正在使用的许多应用。
We have devised a class of isothermal reactions for amplifying DNA. These homogeneous reactions rapidly synthesize short oligonucleotides (8-16 bases) specified by the sequence of an amplification template. Versions of the reactions can proceed in either a linear or an exponential amplification mode. Both of these reactions require simple, constant conditions, and the rate of amplification depends entirely on the molecular parameters governing the interactions of the molecules in the reaction. The exponential version of the reaction is a molecular chain reaction that uses the oligonucleotide products of each linear reaction to create producers of more of the same oligonucleotide. it is a highly sensitive chain reaction that can be specifically triggered by given DNA sequences and can achieve amplifications of >10(6)-fold. Several similar reactions in this class are described here. The robustness, speed, and sensitivity of the exponential reaction suggest it will be useful in rapidly detecting the presence of small amounts of a specific DNA sequence in a sample, and a range of other applications, including many currently making use of the PCR.