ANALYSIS OF THE ENTIRE NUCLEOTIDE-SEQUENCE OF THE CRYPTIC PLASMID OF CHLAMYDIA-TRACHOMATIS SEROVAR L1 - EVIDENCE FOR INVOLVEMENT IN DNA-REPLICATION
ANALYSIS OF THE ENTIRE NUCLEOTIDE-SEQUENCE OF THE CRYPTIC PLASMID OF CHLAMYDIA-TRACHOMATIS SEROVAR L1 - EVIDENCE FOR INVOLVEMENT IN DNA-REPLICATION
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DOI：
10.1093/nar/16.9.4053
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发表时间：
1988-05-11
影响因子：
14.9
通讯作者：
CLARKE, IN
中科院分区：
文献类型：
--
作者：
HATT, C;WARD, ME;CLARKE, IN
沙眼衣原体血清型L1/440/LN具有7498 bp的质粒，命名为pLGV 440。将该质粒在pAT 153的BamH 1位点克隆到大肠杆菌中，并将重组质粒命名为pCTL 1。构建了pCTL 1的限制性内切酶图谱。衣原体质粒的一个片段在E.当置于lacZ基因的上游时，通过链终止法对整个质粒进行测序。从所得到的共有序列中鉴定了开放阅读框以及质粒复制起点的候选者，所述质粒复制起点由22 bp序列的四个完美串联重复序列、富含A：T的序列和可产生34.8kdal产物的开放阅读框组成。通过计算机将开放阅读框的预测多肽产物与所有已报道的蛋白质序列进行比较。一个开放阅读框的预测多肽产物与E.大肠杆菌dnaB蛋白和噬菌体P22基因12的类似产物。
Chlamydia trachomatis serovar L1/440/LN possesses a 7498bp plasmid which was designated pLGV440. The plasmid was cloned at the BamH1 site of pAT153 into Escherichia coli and the recombinant plasmid was designated pCTL1. A detailed restriction endonuclease map of pCTL1 was constructed. A fragment of the chlamydial plasmid was shown to function as a promoter in E. coli when placed upstream of the lacZ gene. The entire plasmid was sequenced by the chain termination method. Open reading frames were identified from the resulting consensus sequence together with a candidate for the plasmid origin of replication consisting of four perfect tandem repeats of a 22bp sequence, an A:T rich sequence and an open reading frame which could generate a 34.8kdal product. The predicted polypeptide products of the open reading frames were compared by computer with all reported protein sequences. Homology of the predicted polypeptide product of an open reading frame to the E. coli dnaB protein and the analogous product of gene 12 of bacteriophage P22 is described.