NORFA, long intergenic noncoding RNA, maintains sow fertility by inhibiting granulosa cell death
NORFA, long intergenic noncoding RNA, maintains sow fertility by inhibiting granulosa cell death
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NORFA，长基因间非编码 RNA，通过抑制颗粒细胞死亡来维持母猪生育力
DOI：
10.1038/s42003-020-0864-x
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发表时间：
2020-03-18
影响因子：
5.9
通讯作者：
Li, Qifa
中科院分区：
文献类型：
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作者：
Du, Xing;Liu, Lu;Li, Qifa
Xing Du等显示长基因间非编码RNA（lincRNA）NORFA通过TGF-β信号传导途径抑制颗粒细胞的凋亡性死亡来维持母猪生育力。长基因间非编码RNA（Long intergenic non-coding RNA，lincRNA）是一种重要的非编码RNA，它参与调控雌性生殖。然而，lincRNA在多大程度上参与卵巢功能和生育力尚不完全清楚。在此我们发现一种lincRNA，NORFA参与了颗粒细胞凋亡，卵泡闭锁和母猪生育力.我们发现NORFA在卵泡闭锁过程中下调，并抑制颗粒细胞凋亡。NORFA直接与miR-126相互作用，从而阻止其与TGFBR 2 3 '-UTR结合。miR-126通过减弱NORFA诱导的TGF-β信号通路促进颗粒细胞凋亡重要的是，在NORFA启动子中检测到一个品种特异性的19-bp重复，证明该重复通过募集转录因子NFIX增强NORFA的转录活性而与母猪生育力相关。综上所述，本研究发现了一个与母猪多育性相关的候选lincRNA，为进一步研究卵泡闭锁和雌性生育力的机制提供了理论依据。
Xing Du et al. show that long intergenic non-coding RNA (lincRNA) NORFA maintains sow fertility by inhibiting apoptotic death of granulosa cells through the TGF-beta signaling pathway. A breed-specific 19-bp duplication suggests that transcription factor NFIX may enhance the transcription of NORFA, providing insights into the role of lincRNAs for female fertility.Long intergenic non-coding RNAs (lincRNAs) have been proved to be involved in regulating female reproduction. However, to what extent lincRNAs are involved in ovarian functions and fertility is incompletely understood. Here we show that a lincRNA, NORFA is involved in granulosa cell apoptosis, follicular atresia and sow fertility. We found that NORFA was down-regulated during follicular atresia, and inhibited granulosa cell apoptosis. NORFA directly interacted with miR-126 and thereby preventing it from binding to TGFBR2 3 '-UTR. miR-126 enhanced granulosa cell apoptosis by attenuating NORFA-induced TGF-beta signaling pathway. Importantly, a breed-specific 19-bp duplication was detected in NORFA promoter, which proved association with sow fertility through enhancing transcription activity of NORFA by recruiting transcription factor NFIX. In summary, our findings identified a candidate lincRNA for sow prolificacy, and provided insights into the mechanism of follicular atresia and female fertility.