A Family of Basic Amino Acid Transporters of the Vacuolar Membrane from Saccharomyces cerevisiae*
A Family of Basic Amino Acid Transporters of the Vacuolar Membrane from Saccharomyces cerevisiae*
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DOI：
10.1074/jbc.m412617200
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发表时间：
2005-02
影响因子：
4.8
通讯作者：
Masamitsu Shimazu;T. Sekito;Koichi Akiyama;Y. Ohsumi;Y. Kakinuma
中科院分区：
文献类型：
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作者：
Masamitsu Shimazu;T. Sekito;Koichi Akiyama;Y. Ohsumi;Y. Kakinuma
在酿酒酵母的主要助酵剂超家族的成员中，我们发现了参与碱性氨基酸组氨酸、赖氨酸和精氨酸转运到液泡中的基因。在液泡碱性氨基酸转运蛋白1 （VBA1）缺失菌株YMR088c中，分离的液泡膜囊对组氨酸和赖氨酸的atp依赖性摄取受损，而对酪氨酸和钙的摄取几乎没有影响。VBA1基因的引入完全弥补了组氨酸和赖氨酸摄取的缺陷，而编码Vba1p与绿色荧光蛋白融合的基因则部分弥补了这一缺陷，该基因被确定只定位于液泡膜。在突变体YBR293w （VBA2）和YCL069w （VBA3）的空泡膜泡中也观察到组氨酸、赖氨酸或精氨酸摄取的缺陷。这三个VBA基因在系统发育上密切相关，构成了酵母液泡中一个新的碱性氨基酸转运蛋白家族。
Among the members of the major facilitator superfamily of Saccharomyces cerevisiae, we identified genes involved in the transport into vacuoles of the basic amino acids histidine, lysine, and arginine. ATP-dependent uptake of histidine and lysine by isolated vacuolar membrane vesicles was impaired in YMR088c, a vacuolar basic amino acid transporter 1 (VBA1)-deleted strain, whereas uptake of tyrosine or calcium was little affected. This defect in histidine and lysine uptake was complemented fully by introducing the VBA1 gene and partially by a gene encoding Vba1p fused with green fluorescent protein, which was determined to localize exclusively to the vacuolar membrane. A defect in the uptake of histidine, lysine, or arginine was also observed in the vacuolar membrane vesicles of mutants YBR293w (VBA2) and YCL069w (VBA3). These three VBA genes are closely related phylogenetically and constitute a new family of basic amino acid transporters in the yeast vacuole.