Transcription analysis on response of porcine alveolar macrophages to co-infection of the highly pathogenic porcine reproductive and respiratory syndrome virus and Mycoplasma hyopneumoniae
Transcription analysis on response of porcine alveolar macrophages to co-infection of the highly pathogenic porcine reproductive and respiratory syndrome virus and Mycoplasma hyopneumoniae
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猪肺泡巨噬细胞对高致病性猪繁殖与呼吸综合征病毒和猪肺炎支原体感染反应的转录分析
DOI：
10.1016/j.virusres.2014.11.006
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发表时间：
2015-01-22
期刊：
影响因子：
5
通讯作者：
He, Kongwang
中科院分区：
文献类型：
--
作者：
Li, Bin;Du, Luping;He, Kongwang
猪呼吸道疾病综合症 (PRDC) 在经济上对全世界的养猪生产者来说都是一个重大问题。猪繁殖与呼吸综合征病毒 (PRRSV) 和猪肺炎支原体 (Mhp) 的共同感染被认为是 PRDC 的主要病原体，并导致猪的大规模死亡。然而，由于缺乏有关共同感染后宿主反应的信息，导致发病机制和持续感染的宿主因素的分子机制尚未明确。在当前的研究中，采用高通量 cDNA 微阵列检测来评估猪肺泡巨噬细胞 (PAM) 对高致病性 PRRSV (HP-PRRSV) 和 Mhp 共感染的宿主反应。感染后6小时和15小时，对照组和PRRSV+Mhp共感染组分别鉴定出总共2152个和1760个基因存在差异表达。 DE基因涉及许多重要的功能类别，包括炎症反应、免疫反应、细胞凋亡、防御反应、信号转导。通路分析表明，最重要的通路与趋化因子信号通路、细胞因子、TLR、RLR和NLR信号通路以及Jak-STAT信号通路相关。 STRING 分析表明，IL-1 beta 是 PRRSV 和 Mhp 联合感染中的一个整合基因。本研究首次记录了 PAM 对 HP-PRRSV 和 Mhp 联合感染的反应。观察到的基因表达谱有助于筛选潜在的宿主因子，以减少联合感染的发生率，并进一步加深我们对猪 PRRSV 和 Mhp 联合感染相关分子发病机制的理解。 (C) 2014 Elsevier B.V. 保留所有权利。
Porcine respiratory disease complex (PRDC) is of great concern economically, for swine producers worldwide. Co-infections with porcine reproductive and respiratory syndrome virus (PRRSV) and Mycoplasma hyopneumoniae (Mhp) are considered the major causative agents of PRDC, and responsible for mass mortality in pigs. Nevertheless, the molecular mechanisms underlying the host factors involved in pathogenesis and persistent infection have not been clearly established because of a lack of information regarding host responses following co-infection. In the current study, high throughput cDNA microarray assays were employed to evaluate host responses of porcine alveolar macrophages (PAM) to co-infection with highly pathogenic PRRSV (HP-PRRSV) and Mhp. A total of 2152 and 1760 genes were identified as being differentially expressed between the control group and PRRSV+Mhp co-infected group at 6 and 15 h post infection, respectively. The DE genes were involved in many vital functional classes, including inflammatory response, immune response, apoptosis, defense response, signal transduction. The pathway analysis demonstrated that the most significant pathways were associated with chemokine signaling pathway, cytokine, TLR, RLR and NLR signaling pathways and Jak-STAT signaling pathway. STRING analysis demonstrated that IL-1 beta is an integral gene in co-infections with PRRSV and Mhp. The present study is the first to document the response of PAMs to co-infection with HP-PRRSV and Mhp. The observed gene expression profile could help with the screening of potential host agents for reducing the prevalence of co-infections, and to further develop our understanding of the molecular pathogenesis associated with PRRSV and Mhp co-infection in pigs. (C) 2014 Elsevier B.V. All rights reserved.