An 11-year linezolid-resistant Staphylococcus capitis clone dissemination with a similar cfr-carrying plasmid in China.
An 11-year linezolid-resistant Staphylococcus capitis clone dissemination with a similar cfr-carrying plasmid in China.
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DOI：
10.1016/j.isci.2022.105644
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发表时间：
2022-12-22
期刊：
影响因子：
5.8
通讯作者：
Cai, Jiachang
中科院分区：
文献类型：
--
作者：
Shen, Weiyi;Chen, Jiawei;Zhang, Rong;Cai, Jiachang
近年来，利奈唑胺耐药性在医院病原体中广泛传播，已成为全球关注的问题。2011 - 2021年在国内某三级医院连续分离到耐利奈唑胺性头炎葡萄球菌（LRSC） 102株，呈单克隆传播。在90株分离株中鉴定出结构相似的cfr携带质粒。在一个菌株（LR95）中，染色体cfr位于tn4001样转座子和ISEnfa4旁边。在11个分离株和体外传代实验中观察到携带cfr的质粒丢失。偶联实验表明，携带cfr的质粒可以水平转移到金黄色葡萄球菌RN4220中。cfr阳性的LRSC与金黄色葡萄球菌的生长速率均无显著差异，只有金黄色葡萄球菌表现出竞争缺陷，说明该质粒对LRSC施加了一定的适应成本。因此，应该采取持续的措施来控制这些抗微生物细菌的传播。携带cfr基因的质粒在MRSA中的不稳定性cfr基因在头型葡萄球菌微生物学中的适应度成本细菌学;进化生物学。
Linezolid resistance has represented a global concern with its wide dissemination among nosocomial pathogens in recent years. One hundred and two linezolid-resistant Staphylococcus capitis (LRSC) were constantly isolated from 2011 to 2021, which demonstrated single clonal dissemination in a Chinese tertiary hospital. A structurally similar cfr-carrying plasmid was identified among 90 isolates. A chromosomal cfr was located beside a Tn4001-like transposon and ISEnfa4 in one strain (LR95). The loss of cfr-carrying plasmid was observed in 11 isolates and the in vitro passage experiments. Conjugation experiments demonstrated the horizontal transferability of the cfr-carrying plasmid into Staphylococcus aureus RN4220. Both cfr-positive LRSC and S. aureus showed no significant differences in growth rates, while only the former displayed competition defect, suggesting this plasmid imposed a certain fitness cost on LRSC. Hence, ongoing measurements are supposed to be adopted to control the spread of these antimicrobial-resistant bacteria. An eleven-year persistence of nosocomial LRSC clone dissemination Similar vector backbones of cfr-carrying plasmids in evolution Instability of cfr-plasmids in MRSA Fitness cost of the carriage of the cfr gene on Staphylococcus capitis Microbiology; Bacteriology; Evolutionary biology.
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