Improvement of cooling rate during cryopreservation of living cells
Improvement of cooling rate during cryopreservation of living cells
复制标题
提高活细胞冷冻保存过程中的冷却速度
DOI：
10.1088/1742-6596/1857/1/012001
复制
发表时间：
2020
期刊：
影响因子：
--
通讯作者：
S. Funaki and N. Savela
中科院分区：
文献类型：
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作者：
M. Nozawa;S. Funaki and N. Savela
低温保存用于长期储存和运输。人类 ES/iPS 细胞可用于再生医学。采用超低温保存和运输人ES/iPS细胞的问题是这些细胞在冻融过程中的存活率较低。需要更好的冷却速率和冷冻保护剂来提高细胞存活率。冰晶的生长引起脱水、变形、收缩和电解质浓度的增加。当冷却得足够快时，细胞会冻结在玻璃化状态，冰晶没有时间形成。为了实现高冷却速率，需要浸入液氮中。为了实现比当前状态更高的冷却速率，pur先前的研究报道，研究了冷冻保存过程中表面状况对冷却速率的影响。确认了通过用不锈钢网覆盖冷却对象，冷却速度提高。然而，薄膜沸腾过程中气泡行为与表面条件的差异尚未阐明。为了研究高冷却速率下的沸腾状态，使用高速相机对冷却物体表面的薄膜沸腾进行可视化。从图像分析结果证实，气泡的尺寸和频率随表面状况而变化。
Cryopreservation is used in long term storage and transportation. Human ES/iPS cells could be useful in regenerative medicine. The problem of using cryopreservation for storing and transporting human ES/iPS cells is that the survival rate of these cells is low during the freezing and thawing process. Better cooling rates and cryoprotectants are needed for improvement of the cell survival rate. The growth of the ice crystals causes dehydration, deformation, contraction and increase of the electrolytic concentration. When cooled fast enough the cells freeze in the vitrification state and the ice crystals don't have time to form. Immersion in liquid nitrogen is necessary to achieve a high cooling rate. To achieve a higher cooling rate than the current state, it was reported in pur previous study that the effect of the surface condition on the cooling rate during cryopreservation was investigated. It was confirmed that the cooling rate is improved by covering the cooling subject with a stainless steel mesh. However, the difference in behavior of the vapor bubble during film boiling with surface condition has not been clarified yet. In order to investigate the boiling state with high cooling rate, visualization of film boiling on the surface of cooling object was performed using a high-speed camera. It was confirmed from the image analysis results that the size and frequency of the vapor bubble were changed with the surface condition.