Grape-seed procyanidins prevent low-grade inflammation by modulating cytokine expression in rats fed a high-fat diet
Grape-seed procyanidins prevent low-grade inflammation by modulating cytokine expression in rats fed a high-fat diet
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DOI：
10.1016/j.jnutbio.2008.02.005
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发表时间：
2009-03-01
影响因子：
5.6
通讯作者：
Blay, Mayte
中科院分区：
文献类型：
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作者：
Terra, Ximena;Montagut, Gemma;Blay, Mayte
目的：本研究的主要目的是评估原花青素摄入量对高脂饮食大鼠炎症介质水平的影响，高脂饮食大鼠是一种低度炎症模型，因为它们显示出细胞因子产生的改变。设计：将雄性Zucker Fa/fa大鼠随机分组以接受低脂肪（LF）饮食，高脂肪（HF）饮食或补充有来自葡萄籽的原花青素（HFPE）（345 mg/kg饲料）的高脂肪饮食19周，然后安乐死。检测血浆生化指标、C-反应蛋白（CRP）和IL-6水平。还测定了脂肪组织贮库和体重。我们评估了CRP，IL-6，TNF-α和脂联素基因在肝脏和脂肪组织（WAT）中的表达。结果：正如预期的那样，喂食HF饮食的大鼠显示出CRP的产生增强。HFPE大鼠血浆CRP的降低与肝脏和肠系膜WAT中CRP mRNA表达的下调有关。我们还发现，肠系膜WAT中促炎细胞因子TNF-α和IL-6的表达减少。与此相反，脂联素mRNA在此组织中增加，由于原花青素treatment.As先前报道，CRP血浆水平呈正相关，其在肠系膜WAT的表达，表明原花青素提取物（PE）调节CRP的合成水平。CRP血浆水平也与体重正相关。正如预期的那样，体重与肥胖指数相关。TNF-α表达与IL-6表达呈正相关。与此相反，抗炎细胞因子脂联素的表达呈负相关的表达TNF-α和IL-6在肠系膜WAT。结论：这些结果表明，PE对低度炎症性疾病的有益作用，这可能与抑制促炎分子CRP，IL-6和TNF-α和抗炎细胞因子脂联素的产生增强。这些发现为探索膳食多酚在减少肥胖相关脂肪因子失调以管理心血管和代谢危险因素方面的作用提供了强大的动力。(C)2009 Elsevier Inc. All rights reserved.
Objective: The main objective of this study was to evaluate the effect of procyanidin intake on the level of inflammatory mediators in rats fed a hyperlipidic diet, which are a model of low-grade inflammation as they show an altered cytokine production.Design: Male Zucker Fa/fa rats were randomly grouped to receive a low-fat (LF) diet, a high-fat (HF) diet or a high-fat diet supplemented with procyanidins from grape seed (HFPE) (345 mg/kg feed) for 19 weeks and were then euthanized. We determined biochemical parameters, C-reactive protein (CRP) and IL-6 levels in plasma. Adipose tissue depots and body weight were also determined. We assessed CRP, IL-6, TNF-alpha and adiponectin gene expression in liver and while adipose tissue (WAT).Results: As expected, rats fed the HF diet show an enhanced production of CRP Our results demonstrate that the HFPE diet decreases rat plasma CRP levels but not IL-6 levels. The decrease in plasma CRP in HFPE rats is related to a down-regulation of CRP mRNA expression in the liver and mesenteric WAT. We have also shown a decrease in the expression of the proinflammatory cytokines TNF-alpha and IL-6 in the mesenteric WAT. In contrast, adiponectin mRNA is increased in this tissue due to the procyanidin treatment.As previously reported, CRP plasma levels correlate positively with its expression in the mesenteric WAT, suggesting that procyanidin extract (PE) modulates CRP at the synthesis level. CRP plasma levels also correlate positively with body weight. As expected, body weight is associated with the adiposity index. Also, TNF-a expression and IL-6 expression have a strong positive correlation. In contrast, the expression of the anti-inflammatory cytokine adiponectin correlates negatively with the expression of TNF-alpha and IL-6 in the mesenteric WAT.Conclusion: These results suggest a beneficial effect of PE on low-grade inflammatory diseases, which may be associated with the inhibition of the proinflammatory molecules CRP, IL-6 and TNF-alpha and the enhanced production of the anti-inflammatory cytokine adiponectin. These findings provide a strong impetus to explore the effects of dietary polyphenols in reducing obesity-related adipokine dysregulation to manage cardiovascular and metabolic risk factors. (C) 2009 Elsevier Inc. All rights reserved.