Masaru Tachibana and Kenichi Kojima Digital topography with an X-ray CCD camera for characterizing perfection in protein crystals
Masaru Tachibana and Kenichi Kojima Digital topography with an X-ray CCD camera for characterizing perfection in protein crystals
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Masaru Tachibana 和 Kenichi Kojima 使用 X 射线 CCD 相机进行数字形貌分析，用于表征蛋白质晶体的完美特征
DOI：
10.1107/s0021889812032049
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发表时间：
2012
影响因子：
6.1
通讯作者：
Mengyuan Shen
中科院分区：
文献类型：
--
作者：
Kei Wako;Kunio Kimura;Yu Yamamoto;Takuya Sawaura;Mengyuan Shen
采用X射线CCD数字X射线形貌术和X射线胶片常规X射线形貌术研究了四棱鸡卵清溶菌酶（HEWL）晶体。以前，蛋白质晶体的清晰位错图像主要通过胶片方法获得。早期的研究HEWL晶体使用X射线CCD相机主要揭示域结构。在本研究中，同一HEWL晶体的位错图像已获得通过使用传统的X射线胶片和数字X射线CCD相机。结果表明，使用X射线CCD相机的数字地形是一种有效的方法来表征蛋白质晶体。通过由Lovelace，Murphy，Pahl，Schmidter & Borgstahl [J. Appl. Cryst.（2006），39，425-432]开发的方法分析一系列数字地形图像。在局部摇摆曲线中观察到位错引起的亚峰和峰展宽。此外，通过绘制局部摇摆曲线的最大值和半峰全宽的角位置来评价晶体的完整性。
Digital X-ray topography using an X-ray CCD camera and conventional X-ray topography using X-ray film were used to investigate tetragonal hen egg-white lysozyme (HEWL) crystals. Previously, clear dislocation images of protein crystals were mainly obtained by film methods. Earlier studies of HEWL crystals using an X-ray CCD camera mainly revealed domain structures. In the present study, dislocation images of the same HEWL crystal have been obtained by using conventional X-ray film and a digital X-ray CCD camera. The results demonstrate that digital topography using an X-ray CCD camera is an effective method for characterizing protein crystals. A series of digital topographic images were analyzed by the method developed by Lovelace, Murphy, Pahl, Brister & Borgstahl [J. Appl. Cryst. (2006), 39, 425–432]. Sub-peaks and peak broadening originating from dislocations in local rocking curves were observed. Moreover, the crystal perfection was evaluated by mapping the angular positions of the maximums and the full widths at half-maximum of local rocking curves.