Genomic amplification of the human telomerase gene (hTERC) associated with human papillomavirus is related to the progression of uterine cervical dysplasia to invasive cancer.
Genomic amplification of the human telomerase gene (hTERC) associated with human papillomavirus is related to the progression of uterine cervical dysplasia to invasive cancer.
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与人乳头瘤病毒相关的人端粒酶基因（HTERC）的基因组扩增与子宫宫颈发育不良对浸润性癌的发展有关。
DOI：
10.1186/1746-1596-7-147
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发表时间：
2012-10-30
影响因子：
2.6
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Liu H;Liu S;Wang H;Xie X;Chen X;Zhang X;Zhang Y
人乳头瘤病毒（HPV）感染在宫颈发育不良和癌症的发展中发挥着病因作用。人类端粒酶基因（hTERC）的扩增和端粒酶的过度表达被发现与宫颈肿瘤的发生有关。本研究旨在分析宫颈上皮内瘤变（CIN）和宫颈癌不同阶段的hTERC基因基因组扩增、端粒酶活性与HPV感染的关系。我们正在研究 hTERC 在宫颈发育不良发展为浸润性癌症中的作用，并提出了一种宫颈癌筛查的辅助方法。从 114 名患有非肿瘤性病变 (NNL，n=27)、宫颈上皮内瘤变 (CIN1，n=26、CIN2，n=16、CIN3，n=24) 和宫颈癌 (CA，n=21) 的患者中收集脱落的宫颈细胞，并使用双色荧光原位杂交 (FISH) 探针和 Hybrid Capture 2 分析 HPV-DNA 的 hTERC 扩增。对 53 名患者进行活检，通过端粒重复扩增方案 (TRAP) 分析端粒酶活性，并通过免疫组织化学 (IHC) 分析人端粒酶逆转录酶 (hTERT) 的表达。所有活检均由病理学家临床证实。 hTERC 的扩增与组织学诊断显着相关（p<0.05）。 hTERC扩增水平与不典型增生的组织学分级呈正相关（CIN1或正常为CIN2/3，P=0.03）。与 CIN2 组相比，CIN3 亚组中 HPV 和 hTERC 阳性病例的积累显着增加，分别为 25% 和 62.96% (p=0.007)。 hTERC 扩增可以通过 FISH 技术对脱落的宫颈细胞进行检测。 hTERC 和 HPV 感染的扩增与更进展的 CIN3 和 CA 相关。 hTERC 扩增检测可能是细胞学筛查和 HPV 检测的补充，尤其是高危患者。本文的虚拟幻灯片可以在这里找到：http://www.diagnosticpathology.diagnomx.eu/vs/1857134686755648。
Human papillomavirus (HPV) infection plays an etiological role in the development of cervical dysplasia and cancer. Amplification of human telomerase gene (hTERC) and over expression of telomerase were found to be associated with cervical tumorigenesis. This study was performed to analyze genomic amplification of hTERC gene, telomerase activity in association with HPV infection in different stages of cervical intraepithelial neoplasia (CIN) and cervical cancer. We were studying the role of hTERC in the progression of uterine cervical dysplasia to invasive cancer, and proposed an adjunct method for cervical cancer screening. Exfoliated cervical cells were collected from 114 patients with non neoplastic lesion (NNL, n=27), cervical intraepithelial neoplasia (CIN1, n=26, CIN2, n=16, CIN3, n=24) and cervical carcinoma (CA, n=21), and analyzed for amplification of hTERC with two-color fluorescence in situ hybridization (FISH) probe and HPV-DNA with Hybrid Capture 2. From these patients, 53 were taken biopsy to analyze telomerase activity by telomeric repeat amplification protocol (TRAP) and expression of human telomerase reverse transcriptase (hTERT), with immunohistochemistry (IHC). All biopsies were clinically confirmed by phathologists. Amplification of hTERC was significantly associated with the histologic diagnoses (p<0.05). The positive correlation was found between the level of hTERC amplification and histologic grading of dysplasia (CIN2/3 from CIN1 or normal, P=0.03). A profounding increase in the accumulation of HPV and hTERC positive cases was observed in the CIN3 subgroup compared with the CIN2 group, 25% versus 62.96%, respectively (p=0.007). hTERC ampliffication can be detected with FISH technique on exfoliated cervical cells. Amplification of hTERC and HPV infection are associated with more progressive CIN3 and CA. The testing of hTERC amplification might be a supplementary to cytology screening and HPV test, especially high-risk patients. The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1857134686755648.
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