Comparative effects of genistein and bisphenol A on non-alcoholic fatty liver disease in laying hens
Comparative effects of genistein and bisphenol A on non-alcoholic fatty liver disease in laying hens
复制标题
金雀异黄素和双酚A对蛋鸡非酒精性脂肪性肝病治疗效果的比较
DOI：
10.1016/j.envpol.2021.117795
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发表时间：
2021-07-16
影响因子：
8.9
通讯作者：
Song, Suquan
中科院分区：
文献类型：
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作者：
Gao, Xiaona;Liu, Shuhui;Song, Suquan
双酚A(BPA)和染料木素(GEN)是选择性雌激素受体调节剂，参与代谢综合征的发生发展。然而，它们在蛋鸡非酒精性脂肪性肝病(NAFLD)中的作用尚未见报道。在此，我们研究了不同浓度的GEN和BPA对蛋鸡NAFLD的影响。结果表明，GEN通过改善高能量低蛋白饮食(HELP)诱导的NAFLD的病理损伤、肝脏脂肪变性和胰岛素抵抗，并阻断NLRP3炎症组相关因子的表达，从而改善高能量低蛋白饮食(HELP)诱导的NAFLD。而大剂量双酚A可加重NAFLD症状加重，并显著抑制肝脏ERα水平，而GEN则呈剂量依赖关系逆转这种现象。总之，我们的研究表明，Gen对NAFLD的保护作用旨在改善与ERα密切相关的代谢紊乱和炎症，而双酚A则可以抑制ERα的表达，加重NAFLD的症状。综上所述，我们阐明了GEN和BPA在蛋鸡NAFLD中的相反作用，从而提供了一个与ERα和炎症相关的潜在机制。
Bisphenol A (BPA) and genistein (GEN) are selective estrogen receptor modulators, which are involved in the occurrence and development of metabolic syndrome. However, their roles in non-alcoholic fatty liver disease (NAFLD) of laying hens have not been reported. Here, we investigated the effects of different concentrations of GEN and BPA on the NAFLD of laying hens. Results showed that GEN ameliorated the high-energy and low-protein diet (HELP)-induced NAFLD by improving pathological damage, hepatic steatosis, and insulin resistance and blocking the expression of NOD-like receptor family pyrin domain containing 3 (NLRP3) inflammasome-related factors. By contrast, high dose of BPA could aggravate these changes with serious symptom of NAFLD and suppress the level of ER alpha in the liver considerably, while GEN could reverse this phenomenon in a dose-dependent manner. In general, our research shows that the protective effect of GEN on NAFLD aims to improve the metabolic disorders and inflammation closely connected to ER alpha, while BPA can inhibit the expression of ER alpha and exacerbate the symptom of NAFLD. In conclusion, we elucidate the opposing effects of GEN and BPA in NAFLD of laying hens, thus providing a potential mechanism related to ER alpha and inflammation.