ELECTROPHORETIC VARIATION IN ADENYLATE KINASE OF NEISSERIA-MENINGITIDIS IS DUE TO INTERSPECIES AND INTRASPECIES RECOMBINATION
ELECTROPHORETIC VARIATION IN ADENYLATE KINASE OF NEISSERIA-MENINGITIDIS IS DUE TO INTERSPECIES AND INTRASPECIES RECOMBINATION
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DOI：
10.1073/pnas.92.23.10535
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发表时间：
1995-11-07
影响因子：
11.1
通讯作者：
SPRATT, BG
中科院分区：
文献类型：
--
作者：
FEIL, E;CARPENTER, G;SPRATT, BG
在原核生物和真核生物中，管家酶从自然种群的电泳变异被认为是由随机的主要是中性突变的积累，在自然可转化的细菌脑膜炎奈瑟氏球菌，我们表明，腺苷酸激酶的电泳迁移率的变化是由于种间和种内重组，而不是突变。腺苷酸激酶基因（adk）的核苷酸序列的分离株，表达的主要慢电泳变体是相当均匀的，在平均1.1%的核苷酸位点的序列不同。表达快速迁移变体的罕见分离株的adk序列彼此相同，但与表达优势变体的菌株的adk基因相比，具有显著的嵌合结构。因此，来自快速变体的序列在基因的前158 bp中与典型的缓慢变体的序列相同，但在基因的其余部分（nt 159-636）中相差8.4%。快速电泳变异体似乎是由脑膜炎球菌基因的大部分被密切相关的奈瑟菌属物种的adk基因的相应区域所取代而产生的。表达具有中等迁移率的电泳变体的adk基因在表达快变体和慢变体的adk基因之间是完美的或几乎完美的重组体。因此，在某些细菌种的管家酶中，降解可能在产生微生物可检测的变异中起主要作用。
In prokaryotic and eukaryotic organisms, the electrophoretic variation in housekeeping enzymes from natural populations is assumed to have arisen by the accumulation of stochastic predominantly neutral mutations, In the naturally transformable bacterium Neisseria meningitidis, we show that variation in the electrophoretic mobility of adenylate kinase is due to inter- and intraspecies recombination rather than mutation. The nucleotide sequences of the adenylate kinase gene (adk) from isolates that express the predominant slow electrophoretic variant were rather uniform, differing in sequence at an average of 1.1% of nucleotide sites. The adk sequences of rare isolates expressing the fast migrating variant were identical to each other but had a striking mosaic structure when compared to the adk genes from strains expressing the predominant variant. Thus the sequence from the fast variants was identical to those of typical slow variants in the first 158 bp of the gene but differed by 8.4% in the rest of the gene (nt 159-636). The fast electrophoretic variant appears to have arisen by the replacement of most of the meningococcal gene with the corresponding region from the adk gene of a closely related Neisseria species. The adk genes expressing the electrophoretic variant with intermediate mobility were perfect, or almost perfect, recombinants between the adk genes expressing the fast and slow variants. Recombination may, therefore, play a major role in the generation of electrophoretically detectable variation in housekeeping enzymes of some bacterial species.