ROLES OF MOTILITY, CHEMOTAXIS, AND PENETRATION THROUGH AND GROWTH IN INTESTINAL MUCUS IN THE ABILITY OF AN AVIRULENT STRAIN OF SALMONELLA-TYPHIMURIUM TO COLONIZE THE LARGE-INTESTINE OF STREPTOMYCIN-TREATED MICE
ROLES OF MOTILITY, CHEMOTAXIS, AND PENETRATION THROUGH AND GROWTH IN INTESTINAL MUCUS IN THE ABILITY OF AN AVIRULENT STRAIN OF SALMONELLA-TYPHIMURIUM TO COLONIZE THE LARGE-INTESTINE OF STREPTOMYCIN-TREATED MICE
复制标题
DOI：
10.1128/iai.56.9.2209-2217.1988
复制
发表时间：
1988-09-01
影响因子：
3.1
通讯作者：
COHEN, PS
中科院分区：
文献类型：
--
作者：
MCCORMICK, BA;STOCKER, BAD;COHEN, PS
此前已表明，具有野生型脂多糖（LPS）的鼠伤寒沙门氏菌无毒株SL5316对链霉素处理的CD - 1小鼠大肠的定殖能力要强得多，运动性也强得多，它与小鼠肠道黏液的结合能力远不如几乎同基因的LPS缺陷型转导子SL5325，但在体外穿透一层肠道黏液的能力却远强于后者。在本研究中，从SL5316中分离出无鞭毛转导子SL5681和无趋化性转导子SL5784，并在链霉素处理的小鼠中测试它们相对于SL5316（粗糙型）的相对定殖能力。此外，还测试了这些沙门氏菌菌株在盲肠肠道黏液和盲肠腔内容物中共同生长的能力、在盲肠黏液中生长后的翻滚和游动活性，以及在体外黏附并穿过盲肠黏液的能力。数据显示，无鞭毛和无趋化性的衍生物在大肠的定殖能力几乎与其亲本相同，但不在盲肠腔内容物中生长，而且在盲肠黏液中生长的菌株失去了翻滚和游动活性。此外，LPS缺陷型菌株与盲肠黏液的黏附能力远强于无鞭毛转导子、无趋化性转导子及其亲本，但穿透黏液的能力却远差于它们。因此，运动性和趋化性似乎在无毒鼠伤寒沙门氏菌菌株在小鼠大肠定殖的能力中不起主要作用，定殖可能需要在盲肠黏液中生长，但不依赖于在盲肠腔内容物中的生长，在盲肠黏液中的生长会抑制鼠伤寒沙门氏菌的运动性，LPS缺陷型突变体对盲肠黏液黏附性的增加以及其穿透盲肠黏液能力的较差可能在其较差的肠道定殖能力中起作用。
Previously, it had been shown that an avirulent strain of Salmonella typhimurium, SL5316, with wild-type lipopolysaccharide (LPS) was a far better colonizer of the streptomycin-treated CD-1 mouse large intestine, was far more motile, did not bind to mouse intestinal mucus nearly as well as, but penetrated through a layer of intestinal mucus in vitro far better than an almost isogenic LPS-deficient transductant, SL5325. In the present investigation, a nonflagellated transductant, SL5681, and a noncemotactic transductant, SL5784, were isolated from SL5316 and tested for relative colonizing ability versus SL5316 (rough) in streptomycin-treated mice. In adition, the Salmonella strains were tested for their ability to grow together in cecal intestinal mucus and in cecal luminal contents, for their tumbling and swimming activities after growth in cecal mucus, and for their ability to adhere to and travel through cecal mucus in vitro. The data show that the nonflagellated and nonchemotactic derivatives colonized large intestine nearly as well as their parent and but did not grow in cecal luminal contents, and that cecal mucus-grown strains lost tumbling and swimming activities. Furthermore, the LPS-deficient strain adhered to cecal mucus far better but penetrated mucus far worse than did the nonflagellated transductant, the nonchemotactic transductant, and the parent. Thus, motility and chemotaxis do not appear to play a major role in the ability of the avirulent S. typhimurium strains to colonize the mouse large intestine, colonization may require growth in cecal mucus but does not depend on growth in cecal luminal contents, growth in cecal mucus inhibits S. typhimurium motility, and increased adhesion of the LPS-deficient mutant to cecal mucus and its poor ability to penetrate cecal mucus may play a role in its poor intestine-colonizing ability.