XENOESTROGENS ALTER MAMMARY-GLAND DIFFERENTIATION AND CELL-PROLIFERATION IN THE RAT
XENOESTROGENS ALTER MAMMARY-GLAND DIFFERENTIATION AND CELL-PROLIFERATION IN THE RAT
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DOI：
10.2307/3432863
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发表时间：
1995-07-01
影响因子：
10.4
通讯作者：
LAMARTINIERE, CA
中科院分区：
文献类型：
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作者：
BROWN, NM;LAMARTINIERE, CA
我们研究了大鼠急性暴露于外源性雌激素后乳腺的分化和细胞增殖。青春期雌性Sprague-Dawley大鼠（6只/组）用己烯雌酚（DES）、染料木素、o，p '-DDT、Aroclor 1221、Aroclor 1254、2，3，7，8-四氯二苯并-对-二恶英（TCDD）或溶剂芝麻油处理1周。最后一次给药后18小时处死动物。乳腺全载片的分析显示，暴露于DES，染料木素和o，p '-DDT导致增强的腺体分化和增加上皮细胞增殖，如增殖细胞核抗原免疫组织化学所测量的。TCDD处理抑制细胞增殖和腺体发育。Aroclor 1221和Aroclor 1254处理对细胞增殖和乳腺发育有轻微但无统计学显著性影响。我们的结论是，DES，染料木黄酮和o，p '-DDT给予青春期大鼠作为形态发生剂;即，它们增加细胞增殖，这促进未分化的末端芽成熟为更分化的小叶末端导管结构。
We investigated mammary glad differentiation and cell proliferation in rats after acute exposure to xenoestrogens. Pubertal female Sprague-Dawley rats (six/groups) were treated for 1 week with diethylstilbestrol (DES), genistein, o,p'-DDT, Aroclor 1221, Aroclor 1254, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), or the vehicle, sesame oil. Animals were killed 18 hr after the last treatment. Analysis of mammary whole-mounts revealed that exposure to DES, genistein and o,p'-DDT resulted in enhanced gland differentiation and increase epithelial cell proliferation as measured by proliferating cell nuclear antigen immunohistochemistry. TCDD treatment inhibited cell proliferation and gland development. Aroclor 1221 and Aroclor 1254 treatments had slight but not statistically significant effects on cell proliferation and mammary gland development. We conclude that DES, genistein, and o,p'-DDT given to pubertal rats act as morphogens; i.e., they increase cell proliferation, which promotes maturation of the undifferentiated terminal end buds to more differentiated lobular terminal ductal structures.